Independent anterograde transport and retrograde cotransport of domain components of myelinated axons
| Autor: | James L. Salzer, Yoko Bekku |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Ankyrins
Cytoplasm Protein domain Vesicular Transport Proteins Biology Endocytosis Axonal Transport Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine Protein Domains Report medicine Animals Nerve Growth Factors Axon Retrograde direction Transport Vesicles Cells Cultured Myelin Sheath 030304 developmental biology Neurons 0303 health sciences Trafficking Vesicle Cell Biology Axons Rats Vesicular transport protein medicine.anatomical_structure nervous system Axoplasmic transport Biophysics Schwann Cells Cotransporter Cell Adhesion Molecules 030217 neurology & neurosurgery Neuroscience |
| Zdroj: | The Journal of Cell Biology |
| ISSN: | 1540-8140 0021-9525 |
| Popis: | The authors demonstrate anterograde vesicular transport of multiple axonal membrane proteins is largely independent, indicating that myelinated axonal domains assemble locally. In contrast, there is substantial retrograde cotransport of these proteins consistent with co-endocytosis as a clearance mechanism. Neurons are highly polarized cells organized into functionally and molecularly distinct domains. A key question is whether the multiprotein complexes that comprise these domains are preassembled, transported, and inserted as a complex or whether their components are transported independently and assemble locally. Here, we have dynamically imaged, in pairwise combinations, the vesicular transport of fluorescently tagged components of the nodes of Ranvier and other myelinated axonal domains in sensory neurons cultured alone or together with Schwann cells at the onset of myelination. In general, most proteins are transported independently in the anterograde direction. In contrast, there is substantial cotransport of proteins from distinct domains in the retrograde direction likely due to coendocytosis along the axon. Early myelination did not substantially change these patterns of transport, although it increased the overall numbers of axonal transport vesicles. Our results indicate domain components are transported in separate vesicles for local assembly, not as preformed complexes, and implicate endocytosis along axons as a mechanism of clearance. |
| Databáze: | OpenAIRE |
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