The MITF paralog tfec is required in neural crest development for fate specification of the iridophore lineage from a multipotent pigment cell progenitor
Autor: | Kleio Petratou, Robert N. Kelsh, James A. Lister, Samantha A. Spencer |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
Pigments
Embryo Nonmammalian Heredity Cellular differentiation Gene regulatory network Epithelium Homozygosity Multipotency Animal Cells Medicine and Health Sciences Materials Zebrafish Heterozygosity Multidisciplinary Basic Helix-Loop-Helix Leucine Zipper Transcription Factors Pigmentation Stem Cells Eukaryota Neural crest Cell Differentiation Animal Models Xanthophore Microphthalmia-associated transcription factor Cell biology Phenotypes Experimental Organism Systems Neural Crest Osteichthyes Larva Physical Sciences Vertebrates Melanocytes Medicine Cellular Types Anatomy RNA Guide Kinetoplastida Research Article Cell type Cell Potency Science Materials Science Biology Research and Analysis Methods Model Organisms Genetics Animals Cell Lineage Chromatophores Progenitor cell Multipotent Stem Cells Organisms Biology and Life Sciences Epithelial Cells Cell Biology Zebrafish Proteins biology.organism_classification Biological Tissue Fish Mutagenesis Animal Studies Zoology Developmental Biology |
Zdroj: | PLoS ONE, Vol 16, Iss 1, p e0244794 (2021) PLoS ONE |
ISSN: | 1932-6203 |
Popis: | Understanding how fate specification of distinct cell-types from multipotent progenitors occurs is a fundamental question in embryology. Neural crest stem cells (NCSCs) generate extraordinarily diverse derivatives, including multiple neural, skeletogenic and pigment cell fates. Key transcription factors and extracellular signals specifying NCSC lineages remain to be identified, and we have only a little idea of how and when they function together to control fate. Zebrafish have three neural crest-derived pigment cell types, black melanocytes, light-reflecting iridophores and yellow xanthophores, which offer a powerful model for studying the molecular and cellular mechanisms of fate segregation. Mitfa has been identified as the master regulator of melanocyte fate. Here, we show that an Mitf-related transcription factor, Tfec, functions as master regulator of the iridophore fate. Surprisingly, our phenotypic analysis oftfecmutants demonstrates that Tfec also functions in the initial specification of all three pigment cell-types, although the melanocyte and xanthophore lineages recover later. We show that Mitfa repressestfecexpression, revealing a likely mechanism contributing to the decision between melanocyte and iridophore fate. Our data are consistent with the long-standing proposal of a tripotent progenitor restricted to pigment cell fates. Moreover, we investigate activation, maintenance and function oftfecin multipotent NCSCs, demonstrating for the first time its role in the gene regulatory network forming and maintaining early neural crest cells. In summary, we build on our previous work to characterise the gene regulatory network governing iridophore development, establishing Tfec as the master regulator driving iridophore specification from multipotent progenitors, while shedding light on possible cellular mechanisms of progressive fate restriction. |
Databáze: | OpenAIRE |
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