The MITF paralog tfec is required in neural crest development for fate specification of the iridophore lineage from a multipotent pigment cell progenitor

Autor: Kleio Petratou, Robert N. Kelsh, James A. Lister, Samantha A. Spencer
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Pigments
Embryo
Nonmammalian
Heredity
Cellular differentiation
Gene regulatory network
Epithelium
Homozygosity
Multipotency
Animal Cells
Medicine and Health Sciences
Materials
Zebrafish
Heterozygosity
Multidisciplinary
Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
Pigmentation
Stem Cells
Eukaryota
Neural crest
Cell Differentiation
Animal Models
Xanthophore
Microphthalmia-associated transcription factor
Cell biology
Phenotypes
Experimental Organism Systems
Neural Crest
Osteichthyes
Larva
Physical Sciences
Vertebrates
Melanocytes
Medicine
Cellular Types
Anatomy
RNA
Guide
Kinetoplastida
Research Article
Cell type
Cell Potency
Science
Materials Science
Biology
Research and Analysis Methods
Model Organisms
Genetics
Animals
Cell Lineage
Chromatophores
Progenitor cell
Multipotent Stem Cells
Organisms
Biology and Life Sciences
Epithelial Cells
Cell Biology
Zebrafish Proteins
biology.organism_classification
Biological Tissue
Fish
Mutagenesis
Animal Studies
Zoology
Developmental Biology
Zdroj: PLoS ONE, Vol 16, Iss 1, p e0244794 (2021)
PLoS ONE
ISSN: 1932-6203
Popis: Understanding how fate specification of distinct cell-types from multipotent progenitors occurs is a fundamental question in embryology. Neural crest stem cells (NCSCs) generate extraordinarily diverse derivatives, including multiple neural, skeletogenic and pigment cell fates. Key transcription factors and extracellular signals specifying NCSC lineages remain to be identified, and we have only a little idea of how and when they function together to control fate. Zebrafish have three neural crest-derived pigment cell types, black melanocytes, light-reflecting iridophores and yellow xanthophores, which offer a powerful model for studying the molecular and cellular mechanisms of fate segregation. Mitfa has been identified as the master regulator of melanocyte fate. Here, we show that an Mitf-related transcription factor, Tfec, functions as master regulator of the iridophore fate. Surprisingly, our phenotypic analysis oftfecmutants demonstrates that Tfec also functions in the initial specification of all three pigment cell-types, although the melanocyte and xanthophore lineages recover later. We show that Mitfa repressestfecexpression, revealing a likely mechanism contributing to the decision between melanocyte and iridophore fate. Our data are consistent with the long-standing proposal of a tripotent progenitor restricted to pigment cell fates. Moreover, we investigate activation, maintenance and function oftfecin multipotent NCSCs, demonstrating for the first time its role in the gene regulatory network forming and maintaining early neural crest cells. In summary, we build on our previous work to characterise the gene regulatory network governing iridophore development, establishing Tfec as the master regulator driving iridophore specification from multipotent progenitors, while shedding light on possible cellular mechanisms of progressive fate restriction.
Databáze: OpenAIRE
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