A wide field fluorescence lifetime imaging system using a light sheet microscope
| Autor: | Philip Birch, Lamar Moore, Xiaofei Li, Rupert Young, Roger Phillips, Chris Chatwin |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Fluorescence-lifetime imaging microscopy Microscope Photon Materials science 010308 nuclear & particles physics business.industry Amplifier Capacitive sensing R1 01 natural sciences QC0350 law.invention 03 medical and health sciences Capacitor 030104 developmental biology Optics law Light sheet fluorescence microscopy 0103 physical sciences Microchannel plate detector business |
| Zdroj: | SPIE Photonics Europe |
| ISSN: | 0277-786X |
| Popis: | Fluorescence lifetime imaging microscopy (FLIM) has allowed scientists to discern information about the chemical properties of biological processes and has become a vital tool in the life sciences and medical research communities. Measuring the spatial lifetime distribution of the fluorophores as well as the intensity distribution enables users to discern vital information about the chemical environment. It however, remains challenging and often involves slow scanning. We present a new microscope system based on light sheet illumination that uses a micro channel plate (MCP) device called a Capacitive Division Imaging Readout (CDIR) which has been developed by Photek Ltd. The device uses an array of capacitors to move the charge site from the MCP to four pre-amplifiers and time-over-threshold discriminators. This camera has the ability to image photons as well as measure the arrival time, enabling high speed FLIM imaging of biological samples. |
| Databáze: | OpenAIRE |
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