Dexmedetomidine preserves the endothelial glycocalyx and improves survival in a rat heatstroke model

Autor: Yoshiki Nakajima, Shingo Kawashima, Hiroshi Makino, Kensuke Kobayashi, Atsushi Kobayashi, Takasumi Katoh, Soichiro Mimuro, Tsunehisa Sato, Matsuyuki Doi
Rok vydání: 2018
Předmět:
Zdroj: Journal of Anesthesia. 32:880-885
ISSN: 1438-8359
0913-8668
DOI: 10.1007/s00540-018-2568-7
Popis: Heatstroke causes systemic inflammation, followed by vascular endothelial damage. The normal vascular endothelium is coated by endothelial glycocalyx (EGCX). Dexmedetomidine (DEX) has an anti-inflammatory effect, but there has been little investigation on the influence of heatstroke on EGCX and the effect of DEX on this condition. Therefore, we examined whether EGCX was disrupted in heatstroke and if DEX improved survival and preserves EGCX. Anesthetized Wistar rats were randomly assigned to three groups: a DEX group treated with DEX (5 µg/kg/h) and 0.9% saline infused continuously at 10 ml/kg/h during heat exposure; a NSS group given 0.9% saline during heat exposure; and a SHAM group given 0.9% saline alone without heat exposure. Heatstroke was induced by exposure to an ambient temperature of 40 °C with relative humidity of 60%. The survival rate was assessed up to 2 h after the start of heat exposure. Plasma levels of syndecan-1 and the thickness of EGCX using electron microscopy were measured when the systolic blood pressure fell to less than 80 mmHg. The survival rate after 2 h of heat exposure was significantly higher in the DEX group compared to the NSS group (89% vs. 22%, P = 0.004). Plasma levels of syndecan-1 were 0.6 ± 1.3, 9.7 ± 5.9, and 2.1 ± 3.4 ng/ml in the SHAM, NSS and DEX groups, respectively (P = 0.013). The thickness of EGCX was significantly higher in the DEX group compared with the NSS group (P = 0.001). EGCX was disrupted in heatstroke, and DEX improved survival and preserved EGCX.
Databáze: OpenAIRE
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