1H, 13C and 15N chemical shift assignment of the stem-loops 5b + c from the 5′-UTR of SARS-CoV-2

Autor: Klara R. Mertinkus, J. Tassilo Grün, Nadide Altincekic, Jasleen Kaur Bains, Betül Ceylan, Jan-Peter Ferner, Lucio Frydman, Boris Fürtig, Martin Hengesbach, Katharina F. Hohmann, Daniel Hymon, Jihyun Kim, Božana Knezic, Mihajlo Novakovic, Andreas Oxenfarth, Stephen A. Peter, Nusrat S. Qureshi, Christian Richter, Tali Scherf, Andreas Schlundt, Robbin Schnieders, Harald Schwalbe, Elke Stirnal, Alexey Sudakov, Jennifer Vögele, Anna Wacker, Julia E. Weigand, Julia Wirmer-Bartoschek, Maria A. Wirtz Martin, Jens Wöhnert
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Biomolecular NMR Assignments, 16
ISSN: 1874-270X
1874-2718
Popis: The ongoing pandemic of the respiratory disease COVID-19 is caused by the SARS-CoV-2 (SCoV2) virus. SCoV2 is a member of the Betacoronavirus genus. The 30 kb positive sense, single stranded RNA genome of SCoV2 features 5'- and 3'-genomic ends that are highly conserved among Betacoronaviruses. These genomic ends contain structured cis-acting RNA elements, which are involved in the regulation of viral replication and translation. Structural information about these potential antiviral drug targets supports the development of novel classes of therapeutics against COVID-19. The highly conserved branched stem-loop 5 (SL5) found within the 5'-untranslated region (5'-UTR) consists of a basal stem and three stem-loops, namely SL5a, SL5b and SL5c. Both, SL5a and SL5b feature a 5'-UUUCGU-3' hexaloop that is also found among Alphacoronaviruses. Here, we report the extensive H-1, C-13 and N-15 resonance assignment of the 37 nucleotides (nts) long sequence spanning SL5b and SL5c (SL5b +c), as basis for further in-depth structural studies by solution NMR spectroscopy.
Biomolecular NMR Assignments, 16
ISSN:1874-270X
ISSN:1874-2718
Databáze: OpenAIRE
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