Dynamics of constitutive heterochromatin: two contrasted kinetics of genome restructuring in early cloned bovine embryos
| Autor: | Gaetan Lehmann, Jean Paul Renard, Nathalie Beaujean, Pierre Adenot, Xavier Vignon, Andrey Pichugin, Christophe Audouard, Daniel Le Bourhis |
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| Přispěvatelé: | Signalisation, noyaux et innovations en cancérologie (UMR8126), Université Paris-Sud - Paris 11 (UP11)-Institut Gustave Roussy (IGR)-Centre National de la Recherche Scientifique (CNRS), Biologie du Développement et Reproduction (BDR), Institut National de la Recherche Agronomique (INRA), Biologie du développement et reproduction (BDR), École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Institut Gustave Roussy (IGR)-Université Paris-Sud - Paris 11 (UP11), Centre National de la Recherche Scientifique (CNRS)-École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA) |
| Rok vydání: | 2010 |
| Předmět: |
Nuclear Transfer Techniques
Embryology Chromosomal Proteins Non-Histone Somatic cell [SDV]Life Sciences [q-bio] Autoantigens Histones 0302 clinical medicine Endocrinology Heterochromatin [SDV.BDD]Life Sciences [q-bio]/Development Biology Cells Cultured ComputingMilieux_MISCELLANEOUS Regulation of gene expression 0303 health sciences Microscopy Confocal 030219 obstetrics & reproductive medicine [SDV.BA]Life Sciences [q-bio]/Animal biology Obstetrics and Gynecology Cellular Reprogramming embryonic structures animal structures Cloning Organism Embryonic Development Fertilization in Vitro Biology Cytoplasmic Granules 03 medical and health sciences Animals Constitutive heterochromatin 030304 developmental biology Cell Nucleus Cloning CENPA [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology Cell Biology Fibroblasts Chromatin Assembly and Disassembly Embryo Mammalian Molecular biology Embryonic stem cell Kinetics Reproductive Medicine Chromobox Protein Homolog 5 Oocytes Cattle Heterochromatin protein 1 Centromere Protein B Centromere Protein A |
| Zdroj: | Reproduction Reproduction, BioScientifica, 2010, 139 (1), pp.129-137. ⟨10.1530/REP-08-0435⟩ |
| ISSN: | 1741-7899 1470-1626 |
| Popis: | International audience; Efficient reprograming of the donor cell genome in nuclear transfer (NT) embryos is linked to the ability of the embryos to sustain full-term development. As the nuclear architecture has recently emerged as a key factor in the regulation of gene expression, we questioned whether early bovine embryos obtained from transfer of cultured fibroblasts into enucleated oocytes would adopt an embryo-like nuclear organization. We studied the dynamics of constitutive heterochromatin in the stages prior to embryonic genome activation by distribution analysis of heterochromatin protein CBX1 (HP1), centromeric proteins CENPA and CENPB, and histone H3 three-methylated at lysine 9. Then we applied descriptive, quantitative, and co-localization analyses. A dramatic reorganization of heterochromatic blocks of somatic donor cells was first observed in the late one-cell stage NT embryos. Then at two- and four-cell stages, we found two types of NT embryos: one displaying noncondensed heterochromatin patches similar to IVF embryos, whereas the second type displayed condensed heterochromatin blocks, normally observed in IVF embryos only after the eight-cell stage. These analyses discriminate for the first time two contrasted types of nuclear organization in NT embryos, which may correspond to different functional states of the nuclei. The relationship with the somatic nucleus reprograming efficiency is discussed. |
| Databáze: | OpenAIRE |
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