Involvement of CD8+T Cells in Delayed-type Hypersensitivity Responses against Bovine Leukemia Virus (BLV) Induced in Sheep Vaccinated with Recombinant Vaccinia Virus Expressing BLV Envelope Glycoprotein
| Autor: | S. Ikeyama, T. Chiba, Masanobu Sugimoto, K. Ohishi, W. C. Davis, Yoji Ikawa, Kosuke Okada, Shigeru Numakunai, Kan-ichi Ohshima, H. Suzuki, K. Murakami |
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| Rok vydání: | 1993 |
| Předmět: |
0301 basic medicine
040301 veterinary sciences medicine.drug_class T-Lymphocytes viruses Vaccinia virus Biology Monoclonal antibody 0403 veterinary science 03 medical and health sciences Viral Envelope Proteins Dermis medicine Animals Cytotoxic T cell Hypersensitivity Delayed Skin chemistry.chemical_classification Vaccines Synthetic Sheep General Veterinary Bovine leukemia virus Viral Vaccines 04 agricultural and veterinary sciences Enzootic Bovine Leukosis medicine.disease biology.organism_classification Virology 030104 developmental biology medicine.anatomical_structure chemistry Immunohistochemistry Cattle Glycoprotein Infiltration (medical) CD8 |
| Zdroj: | Veterinary Pathology. 30:104-110 |
| ISSN: | 1544-2217 0300-9858 |
| DOI: | 10.1177/030098589303000202 |
| Popis: | Delayed-type hypersensitivity responses against bovine leukemia virus (BLV) envelope glycoprotein (gp60) were induced in the skin of sheep vaccinated with recombinant vaccinia virus (RVV) expressing BLV glycoprotein. The lesions were characterized by marked infiltration of lymphocytes, slight migration of neutrophils, eosinophils, and macrophages in the dermis to hypodermis, and partial intercellular edema in the reticular layer. Immunohistochemical analysis with monoclonal antibodies demonstrated that the lymphocytic infiltrates consisted mainly of CD8+T cells (53.7–55.8% at 48 hours post-challenge of BLV), CD4+T cells (24.7–26.7%), and B cells (11.5–16.9%). The role of CD4+and CD8+T cells in suppressing BLV growth in RVV-vaccinated animals is discussed. |
| Databáze: | OpenAIRE |
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