Developing functional markers for vitamin E biosynthesis in oil palm

Autor: Xiwei Sun, Haikuo Fan, Dongyi Huang, Yong Xiao, Yajing Dou, Wei Xia, Annaliese S. Mason
Jazyk: angličtina
Rok vydání: 2021
Předmět:
medicine.medical_treatment
Single Nucleotide Polymorphisms
Organic chemistry
Artificial Gene Amplification and Extension
Palm Oil
Biochemistry
Polymerase Chain Reaction
chemistry.chemical_compound
Plant Products
Vitamin E
Cluster Analysis
Oil Palm
Food science
Molecular breeding
Multidisciplinary
food and beverages
Eukaryota
Software Engineering
Agriculture
Vitamins
Plants
Physical sciences
Chemistry
Experimental Organism Systems
Medicine
Engineering and Technology
Tocotrienol
Research Article
Computer and Information Sciences
Science
Arabidopsis Thaliana
Single-nucleotide polymorphism
Brassica
Biology
Biosynthesis
Research and Analysis Methods
Vegetable Oils
Polymorphism
Single Nucleotide
Computer Software
Chemical compounds
Model Organisms
Plant and Algal Models
Genetic variation
Organic compounds
medicine
Genetics
SNP
Indel
Molecular Biology Techniques
Gene
Molecular Biology
Organisms
Biology and Life Sciences
Agronomy
chemistry
Animal Studies
Multiplex Polymerase Chain Reaction
Crop Science
Zdroj: PLoS ONE
PLoS ONE, Vol 16, Iss 11, p e0259684 (2021)
PLoS ONE, Vol 16, Iss 11 (2021)
ISSN: 1932-6203
Popis: Vitamin E is essential for human health and plays positive roles in anti-oxidation. Previously, we detected large variation in vitamin E content among 161 oil palm accessions. In this study, twenty oil palm accessions with distinct variation in vitamin E contents (171.30 to 1 258.50 ppm) were selected for genetic variation analysis and developing functional markers associated with vitamin E contents. Thirty-seven homologous genes in oil palm belonging to vitamin E biosynthesis pathway were identified via BLASTP analysis, the lengths of which ranged from 426 to 25 717 bp (average 7 089 bp). Multiplex PCR sequencing for the 37 genes found 1 703 SNPs and 85 indels among the 20 oil palm accessions, with 226 SNPs locating in the coding regions. Clustering analysis for these polymorphic loci showed that the 20 oil palm accessions could be divided into five groups. Among these groups, group I included eight oil palm accessions whose vitamin E content (mean value: 893.50 ppm) was far higher than other groups (mean value 256.29 to 532.94 ppm). Correlation analysis between the markers and vitamin E traits showed that 134 SNP and 7 indel markers were significantly (p < 0.05) related with total vitamin E content. Among these functional markers, the indel EgTMT-1-24 was highly correlated with variation in vitamin E content, especially tocotrienol content. Our study identified a number of candidate function associated markers and provided clues for further research into molecular breeding for high vitamin E content oil palm.
Databáze: OpenAIRE
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