Mutations in the α-Tubulin 67C Gene Specifically Impair Achiasmate Segregation in Drosophila melanogaster
| Autor: | Kimberly J. Greer, Lisa G. Messina, R. Scott Hawley, M.Y. Walker, Ruria Namba, Heinrich J.G. Matthies |
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| Rok vydání: | 1999 |
| Předmět: |
X Chromosome
Centromere Gene Dosage Kinesins Genes Insect Spindle Apparatus kinesin Chromosome segregation Nondisjunction Genetic Meiosis Tubulin Chromosome Segregation meiosis Animals Drosophila Proteins chromosome Prometaphase Genes Dominant Genetics Models Genetic biology Kinetochore Brief Report meiotic spindle Cell Biology centromeres biology.organism_classification Chromatin Drosophila melanogaster Nondisjunction Mutation Microtubule Proteins Oocytes Female |
| Zdroj: | The Journal of Cell Biology |
| ISSN: | 1540-8140 0021-9525 |
| DOI: | 10.1083/jcb.147.6.1137 |
| Popis: | Drosophila melanogaster oocytes heterozygous for mutations in the α-tubulin 67C gene (αtub67C) display defects in centromere positioning during prometaphase of meiosis I. The centromeres do not migrate to the poleward edges of the chromatin mass, and the chromatin fails to stretch during spindle lengthening. These results suggest that the poleward forces acting at the kinetochore are compromised in the αtub67C mutants. Genetic studies demonstrate that these mutations also strongly and specifically decrease the fidelity of achiasmate chromosome segregation. Proper centromere orientation, chromatin elongation, and faithful segregation can all be restored by a decrease in the amount of the Nod chromokinesin. These results suggest that the accurate segregation of achiasmate chromosomes requires the proper balancing of forces acting on the chromosomes during prometaphase. |
| Databáze: | OpenAIRE |
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