Nuclear envelope-vacuole contacts mitigate nuclear pore complex assembly stress
| Autor: | Susan R. Wente, Christopher L. Lord |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Atg1
Saccharomyces cerevisiae Proteins Saccharomyces cerevisiae Receptors Cytoplasmic and Nuclear Vacuole Biology Article Intermediate Filament Proteins Lipid droplet otorhinolaryngologic diseases Nuclear pore Organelles Membrane and Lipid Biology Cell Biology biology.organism_classification Cell biology Nuclear Pore Complex Proteins stomatognathic diseases Metabolism Vacuoles Nuclear Pore Nucleoporin Nuclear transport Biogenesis Gene Deletion |
| Zdroj: | The Journal of Cell Biology |
| ISSN: | 1540-8140 |
| Popis: | How cells respond to deleterious effects imposed by disrupted nuclear pore complex (NPC) assembly is not well defined. Lord and Wente demonstrate that nuclear envelope–vacuole interactions expand in response to perturbed NPC assembly to promote viability, nuclear envelope remodeling, and proper NPC biogenesis. The intricacy of nuclear pore complex (NPC) biogenesis imposes risks of failure that can cause defects in nuclear transport and nuclear envelope (NE) morphology; however, cellular mechanisms used to alleviate NPC assembly stress are not well defined. In the budding yeast Saccharomyces cerevisiae, we demonstrate that NVJ1- and MDM1-enriched NE–vacuole contacts increase when NPC assembly is compromised in several nup mutants, including nup116ΔGLFG cells. These interorganelle nucleus–vacuole junctions (NVJs) cooperate with lipid droplets to maintain viability and enhance NPC formation in assembly mutants. Additionally, NVJs function with ATG1 to remodel the NE and promote vacuole-dependent degradation of specific nucleoporins in nup116ΔGLFG cells. Importantly, NVJs significantly improve the physiology of NPC assembly mutants, despite having only negligible effects when NPC biogenesis is unperturbed. These results therefore define how NE–vacuole interorganelle contacts coordinate responses to mitigate deleterious cellular effects caused by disrupted NPC assembly. |
| Databáze: | OpenAIRE |
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