THYROID HORMONE ACTION: DEMONSTRATION OF PUTATIVE NUCLEAR RECEPTORS IN HUMAN LYMPHOCYTES
| Autor: | Jir S. Tsai, Herbert H. Samuels |
|---|---|
| Rok vydání: | 1974 |
| Předmět: |
Adult
medicine.medical_specialty Time Factors Endocrinology Diabetes and Metabolism Clinical Biochemistry Receptors Cell Surface Biology Biochemistry Cell Line Iodine Radioisotopes Thyroid hormone receptor beta Endocrinology Internal medicine medicine Animals Humans Pituitary Neoplasms Lymphocytes Binding site Binding Sites Thyroid hormone receptor Triiodothyronine Biochemistry (medical) DNA Rats Dissociation constant Cell nucleus medicine.anatomical_structure Thyroid hormone receptor alpha Nuclear receptor Female |
| Zdroj: | The Journal of Clinical Endocrinology & Metabolism. 38:919-922 |
| ISSN: | 1945-7197 0021-972X |
| DOI: | 10.1210/jcem-38-5-919 |
| Popis: | Lymphocytes isolated from hypothyroid patients contain high-affinity, limited-capacity, nuclear binding sites for triiodothyronine (T3). The estimated equilibrium dissociation constant was 3.08 × 10−11M. This value is virtually identical to that determined with GH1 cells, a rat pituitary tumor cell line, in culture. The maximal binding capacity was estimated to be 4 × 10−15 moles of T3 bound per 100 ug of lymphocyte DNA. This is equivalent to approximately 300 molecules of T3 bound per cell nucleus. |
| Databáze: | OpenAIRE |
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