Development and evaluation of a reverse transcription loop-mediated isothermal amplification assay for rapid detection of a new SFTS bunyavirus
| Autor: | Wen Zhang, Lanzheng Liu, Guoliang Yang, Yuandong Liu, Bing Li, Weichu Huang |
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| Rok vydání: | 2011 |
| Předmět: |
Detection limit
Phlebovirus China Diagnostic methods Time Factors Staining and Labeling Loop-mediated isothermal amplification General Medicine Biology medicine.disease Bunyaviridae Infections Rapid detection Virology Molecular biology Sensitivity and Specificity Fluorescence SFTS bunyavirus Severe fever with thrombocytopenia syndrome Molecular Diagnostic Techniques Clinical diagnosis medicine Humans Reverse Transcription Loop-mediated Isothermal Amplification Nucleic Acid Amplification Techniques |
| Zdroj: | Archives of virology. 157(9) |
| ISSN: | 1432-8798 |
| Popis: | The etiological agent of severe fever with thrombocytopenia syndrome (SFTS) is a bunyavirus that was first identified in China in 2009. We have developed and validated a one-step, single-tube, reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay for detection of SFTS bunyavirus (SFTSV). This assay demonstrated high specificity and sensitivity, with a detection limit of 10(1) TCID(50) ml(-1). When combined with the fluorescent detection reagent (FDR) method, results could be determined by observing a color change within 30 min. As an accurate, rapid, simple and low-cost diagnostic method, this RT-LAMP assay will be helpful for detecting and preventing further SFTSV infection in China. |
| Databáze: | OpenAIRE |
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