Vitamin C acts as a hepatoprotectant in carbofuran treated rat liver slices in vitro

Autor: Ansari, Bechan Sharma, Vivek Kumar Gupta, Sunil Kumar Jaiswal, Nikhat J. Siddiqi
Rok vydání: 2017
Předmět:
0301 basic medicine
Antioxidant
Health
Toxicology and Mutagenesis
medicine.medical_treatment
Pharmacology
Toxicology
medicine.disease_cause
Lipid peroxidation
chemistry.chemical_compound
NaCl
sodium chloride
0302 clinical medicine
biology
MgSO4
magnesium sulfate
EDTA
Ethylenediaminetetraacetic acid
Biochemistry
Catalase
030220 oncology & carcinogenesis
Carbofuran
WME
Williams medium E
NaOH
sodium hydroxide and MDA Malonaldialdehyde
TBA
thiobarbituric acid
TCA
trichloroacetic acid
Article
RNS
reactive nitrogen species
Superoxide dismutase
03 medical and health sciences
ROS
reactive oxygen species
BSA
Bovine serum albumin
In vitro
lcsh:RA1190-1270
SOD
superoxide dismutase
NADH
nicotinamide adenine dinucleotide
medicine
DMSO
Dimethylsulfoxide
lcsh:Toxicology. Poisons
ComputingMethodologies_COMPUTERGRAPHICS
CaCl2
calcium chloride
Vitamin C
KRHB
Krebs-Ringer HEPES Buffer
Hepatotoxicity
CuSO4
copper sulphate
030104 developmental biology
chemistry
Hepatoprotection
Oxidative stress
biology.protein
KCl
potassium chloride
Zdroj: Toxicology Reports
Toxicology Reports, Vol 4, Iss, Pp 265-273 (2017)
ISSN: 2214-7500
DOI: 10.1016/j.toxrep.2017.06.001
Popis: Graphical abstract
Highlights The observations of liver slices when treated with different concentrations of carbofuran were as follows:- 1. increased LPO 2. decreased SOD, CAT, & protein content in all the treatments The observations of liver slices when treated with different concentrations of carbofuran along with vitamin C were as follows:- 1. the levels of LPO, SOD, CAT & total protein content reinstated towards normal level only in liver slices treated with low concentration 2. at higher concentration of carbofuran treatment Vitamin C does not ameliorate the hepatic toxicity induced by carbofuran The in vitro liver slice culture may prove to be a useful model for hepatotoxicological studies and Vitamin C, as a hepatoprotectant in mammalian system.
Carbamates, most commonly used pesticides in agricultural practices, have been reported to produce free radicals causing deleterious effects in animals. The present study was designed to assess the carbofuran induced oxidative stress in rat liver slices in vitro and also to evaluate protective role of vitamin C by incubating them in Krebs-Ringer HEPES Buffer (KRHB) containing incubation media (Williams medium E (WME) supplemented with glucose and antibiotics) with different concentrations of carbofuran. The results demonstrated that carbofuran caused significant increase in lipid peroxidation and inhibition in the activity of hepatic superoxide dismutase (SOD) in concentration dependent manner. The data with incubation medium reflected that carbofuran at lowest concentration caused an increase in SOD activity followed by its inhibition at higher concentration. Carbofuran treatment caused inhibition in the activity of catalase in liver slices and WME incubation medium. Pre-incubation of liver slices and the WME media with vitamin C restored the values of biochemical indices tested. The results indicated that carbofuran might induce oxidative stress in hepatocytes. The pretreatment with vitamin C may offer hepatoprotection from toxicity of pesticide at low concentration only.
Databáze: OpenAIRE
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