Molecular Detection for Japanese Encephalitis and Chikungunya Virus as Etiology Agent for Dengue-Like Fever Symptoms
| Autor: | Tjahjani Mirawati Sudiro, Beti Ernawati Dewi, Rizka Ariani |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Serotype viruses 030106 microbiology Dengue virus medicine.disease_cause Virus Dengue fever reverse transcription polymerase chain reaction 03 medical and health sciences dengue japanese encephalisitis virus 0302 clinical medicine medicine 030212 general & internal medicine Chikungunya chikungunya virus lcsh:R5-920 business.industry virus diseases Japanese encephalitis medicine.disease Virology Reverse transcription polymerase chain reaction Real-time polymerase chain reaction arboviruses Cardiology and Cardiovascular Medicine business lcsh:Medicine (General) |
| Zdroj: | Makara Journal of Health Research, Vol 22, Iss 3, Pp 142-146 (2018) |
| ISSN: | 2356-3656 2356-3664 |
| Popis: | Background : Acute fever with dengue-like fever symptoms with headache, rash, arthritis, perifer bleeding like ptechie and rinhorrhea are symptoms that often complained by patient and it may be caused by arboviruses infection. Arboviruses are very endemic in Indonesia, especially Dengue Virus (DENV), Japanese Encephalisitis Virus (JEV), and Chikungunya Virus (CHIKV) that causing similar symptoms. Molecular detection is very important to confirm the etiology for adequate management. Methods : This study investigated the etiological agent using one step real time RT reverse transcription polymerase chain reaction (RT PCR) method and SYBR Green I as fluorescent binding dye reporter. The viruses that were investigated were JEV and CHIKV. Primer design was using online software NCBI. Those primers fulfill the good primer requirements and can be used as material in RT PCR reaction. Results : The optimum temperature for all primers were at 60 °C. The limit detection of JEV primer was 4355 copy DNA every reaction. Cross reactivity between all primers with DENV serotype 2 RNA and false positive result using healthy person sample were not found. Conclusions : This study has optimized condition RT PCR protocol that can be used as diagnostic tools for patient with dengue-like fever symptoms. |
| Databáze: | OpenAIRE |
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