Babesiosis caused by Babesia vogeli in dogs from Uberlândia State of Minas Gerais, Brazil
| Autor: | Yaarit Nachum-Biala, Gad Baneth, Maria Júlia Rodrigues da Cunha, Natália M. N. Fava, Jaqueline Rosa Garcia, Márcia Cristina Cury, Camila Oliveira Silva Barbosa, Douglas Alves Pereira |
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| Rok vydání: | 2020 |
| Předmět: |
Male
Veterinary medicine medicine.medical_specialty Babesia vogeli 030231 tropical medicine Babesia Polymerase Chain Reaction 030308 mycology & parasitology 03 medical and health sciences Dogs Ticks 0302 clinical medicine Medical microbiology Babesiosis parasitic diseases Prevalence medicine Animals Dog Diseases DNA Primers 0303 health sciences Venipuncture General Veterinary biology General Medicine medicine.disease biology.organism_classification Sequence identity Radial vein Infectious Diseases Blood smear Insect Science Female Parasitology Brazil |
| Zdroj: | Parasitology Research. 119:1173-1176 |
| ISSN: | 1432-1955 0932-0113 |
| Popis: | Babesia is tick-transmitted protozoan parasites that infect mammalian hosts and have a major impact on farm and pet health-associated costs worldwide. This study aimed to test the prevalence of Babesia spp. infection in a small cohort of dogs at a veterinary hospital and to perform molecular characterization of the Babesia species causing the infection. For the PCR assay, 5 mL of blood was collected by venipuncture of the cephalic or radial veins in 300 dogs of different ages, sex, and breeds, which were presented to the veterinary hospital of the Federal University of Uberlândia between March 2015 and April 2016. In addition, a drop of blood was collected from the marginal blood vessels of the ear of dogs included in this study. Ninety-two (30.67%) were positive for Babesia spp., as determined by microscopic observation of the blood smear, revealing the presence of intra-erythrocyte merozoites. For molecular characterization by PCR, 17 samples were chosen from dogs who were tested positive for Babesia spp. by blood smears. Among them, B. vogeli was found to infect all 17 dogs, as determined by 99-100% sequence identity (closest GenBank match KT246307) using primers PIRO A/PIRO B. Our results indicate that the species observed in these dogs was B. vogeli. |
| Databáze: | OpenAIRE |
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