Evolutionary History and Activity of RNase H1-Like Proteins in Arabidopsis thaliana

Autor:	Sebastian Chamera, Pragya Khurana, Sho Fujii, Aleksandra Kmera, Marcin Nowotny, Jan Kuciński, M. Jordan Rowley, Andrzej T. Wierzbicki
Rok vydání:	2020
Předmět:	0106 biological sciences 0301 basic medicine Chloroplasts Physiology Base pair RNase P Ribonuclease H Arabidopsis Plant Science Biology 01 natural sciences Evolution Molecular 03 medical and health sciences chemistry.chemical_compound Nucleic Acids Gene duplication Arabidopsis thaliana RNase H Gene Phylogeny Genetics Arabidopsis Proteins Regular Papers RNA food and beverages Cell Biology General Medicine biology.organism_classification 030104 developmental biology chemistry Brassicaceae biology.protein DNA 010606 plant biology & botany
Zdroj:	Plant and Cell Physiology Plant Cell Physiol
ISSN:	0032-0781
DOI:	10.1093/pcp/pcaa040
Popis:	RNase H1 is an endonuclease specific toward the RNA strand of RNA:DNA hybrids. Members of this protein family are present in most living organisms and are essential for removing RNA that base pairs with DNA. It prevents detrimental effects of RNA:DNA hybrids and is involved in several biological processes. Arabidopsis thaliana has been previously shown to contain three genes encoding RNase H1 proteins that localize to three distinct cellular compartments. We show that these genes originate from two gene duplication events. One occurred in the common ancestor of dicots and produced nuclear and organellar RNase H1 paralogs. Second duplication occurred in the common ancestor of Brassicaceae and produced mitochondrial- and plastid-localized proteins. These proteins have the canonical RNase H1 activity, which requires at least four ribonucleotides for endonucleolytic digestion. Analysis of mutants in the RNase H1 genes revealed that the nuclear RNH1A and mitochondrial RNH1B are dispensable for development under normal growth conditions. However, the presence of at least one organellar RNase H1 (RNH1B or RNH1C) is required for embryonic development. The plastid-localized RNH1C affects plastid DNA copy number and sensitivity to replicative stress. Our results present the evolutionary history of RNH1 proteins in A. thaliana, demonstrate their canonical RNase H1 activity and indicate their role in early embryonic development.
Databáze:	OpenAIRE
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