Detection of herbicide effects on pigment composition and PSII photochemistry in Helianthus annuus by Raman spectroscopy and chlorophyll a fluorescence
| Autor: | Kateřina Novotná, Petr Vítek, Barbora Rapantová, Karel Klem, Petra Hodaňová |
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| Rok vydání: | 2017 |
| Předmět: |
Chlorophyll
0106 biological sciences Flavonols Photosystem II macromolecular substances Spectrum Analysis Raman Photosynthesis Photochemistry 01 natural sciences Fluorescence Plant Epidermis Analytical Chemistry Mesotrione chemistry.chemical_compound Helianthus annuus Clomazone Instrumentation Carotenoid Chlorophyll fluorescence Spectroscopy chemistry.chemical_classification Herbicides Chlorophyll A Photosystem II Protein Complex food and beverages 04 agricultural and veterinary sciences Reference Standards Atomic and Molecular Physics and Optics Plant Leaves chemistry 040103 agronomy & agriculture Helianthus 0401 agriculture forestry and fisheries 010606 plant biology & botany |
| Zdroj: | Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 170:234-241 |
| ISSN: | 1386-1425 |
| DOI: | 10.1016/j.saa.2016.07.025 |
| Popis: | The effects of herbicides from three mode-of-action groups - inhibitors of protoporphyrinogen oxidase (carfentrazone-ethyl), inhibitors of carotenoid biosynthesis (mesotrione, clomazone, and diflufenican), and inhibitors of acetolactate synthase (amidosulfuron) - were studied in sunflower plants (Helianthus annuus). Raman spectroscopy, chlorophyll fluorescence (ChlF) imaging, and UV screening of ChlF were combined to evaluate changes in pigment composition, photosystem II (PSII) photochemistry, and non-photochemical quenching in plant leaves 6d after herbicide application. The Raman signals of phenolic compounds, carotenoids, and chlorophyll were evaluated and differences in their intensity ratios were observed. Strongly augmented relative content of phenolic compounds was observed in the case of amidosulfuron-treated plants, with a simultaneous decrease in the chlorophyll/carotenoid intensity ratio. The results were confirmed by in vivo measurement of flavonols using UV screening of ChlF. Herbicides from the group of carotenoid biosynthesis inhibitors significantly decreased both the maximum quantum efficiency of PSII and non-photochemical quenching as determined by ChlF. Resonance Raman imaging (mapping) data with high resolution (150,000-200,000 spectra) are presented, showing the distribution of carotenoids in H. annuus leaves treated by two of the herbicides acting as inhibitors of carotenoid biosynthesis (clomazone or diflufenican). Clear signs were observed that the treatment induced carotenoid depletion within sunflower leaves. The depletion spatial pattern registered differed depending on the type of herbicide applied. |
| Databáze: | OpenAIRE |
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