Sensitive and simplified method for the differential determination of serum levels of ketone bodies
| Autor: | Y. Ichikawa, Yukio Shigeta, Shizuo Uno, Yutaka Harano, T. Hyosu, K. Kosugi |
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| Rok vydání: | 1983 |
| Předmět: |
Clinical Biochemistry
Hydroxybutyrates Dehydrogenase Ketone Bodies Biochemistry Acetoacetates Absorbance chemistry.chemical_compound Spectrophotometry Diabetes Mellitus medicine Humans 3-Hydroxybutyric Acid Perchloric acid chemistry.chemical_classification Diazonium Compounds Chromatography Azo compound medicine.diagnostic_test Biochemistry (medical) General Medicine chemistry Ketone bodies Spectrophotometry Ultraviolet |
| Zdroj: | Clinica Chimica Acta. 134:327-336 |
| ISSN: | 0009-8981 |
| Popis: | A highly sensitive and simplified method for the differential determination of serum ketone bodies has been developed. Serum was deproteinized with perchloric acid, and acetoacetate contained in the supernate was reacted with newly synthesized p-nitrobenzene diazonium fluoroborate at 37 degrees C for 10 min. The formed hydrazo compound was converted by alkali to the more stable azo compound which has a peak absorbance at 645 nm. For the determination of 3-hydroxybutyrate, this was enzymatically converted to acetoacetate using 3-hydroxybutyrate dehydrogenase, LDH, NAD and pyruvate. Using 0.2 ml serum, acetoacetate and 3-hydroxybutyrate could be quantitated in 30 min. The described method is five times more sensitive than the enzymatic photometric method and can detect individual ketone bodies at concentrations as low as 20 mumol/l. Differential determination of serum levels of ketone bodies is clinically useful for the diagnosis of type 1 diabetes and in monitoring diabetic control. |
| Databáze: | OpenAIRE |
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