A European multicenter evaluation study to investigate the performance on commercially available selective agar plates for the detection of carbapenemase producing Enterobacteriaceae
| Autor: | Cindy Dierikx, Stefan Börjesson, Agnès Perrin-Guyomard, Marisa Haenni, Madelaine Norström, Hege H. Divon, Hanna Karin Ilag, Sophie A. Granier, Annette Hammerum, Jette Sejer Kjeldgaard, Natalie Pauly, Luke Randall, Muna F. Anjum, Aleksandra Smialowska, Alessia Franco, Kees Veldman, Jannice Schau Slettemeås |
|---|---|
| Přispěvatelé: | National Institute for Public Health and the Environment [Bilthoven] (RIVM), Public Health Agency of Sweden, Laboratoire de Fougères - ANSES, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Laboratoire de Lyon [ANSES], Université de Lyon-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Norwegian Veterinary Institute [Oslo], Statens Serum Institut [Copenhagen], Danmarks Tekniske Universitet = Technical University of Denmark (DTU), German Federal Institute for Risk Assessment [Berlin] (BfR), Animal and Plant Health Agency [Addlestone, UK] (APHA), National Veterinary Research Institute [Pulawy, Pologne] (NVRI), Istituto Zooprofilattico Sperimentale del Lazio e della Toscana (IZSLT), Wageningen BioVeterinary Research, Wageningen University and Research [Wageningen] (WUR), European Project: 773830, H2020-SFS-2017-1 ,One Health EJP(2018) |
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
antibiotic resistance
Selective agar Swine résistance aux antibiotiques Salmonella [SDV.IDA]Life Sciences [q-bio]/Food engineering ddc:630 animal résistance MESH: Enterobacteriaceae Infections MESH: Swine bactérie 2. Zero hunger MESH: Microbial Sensitivity Tests 0303 health sciences Bacteriologie Enterobacteriaceae Infections Bacteriology Host Pathogen Interaction & Diagnostics IMPART Carbapenemases Text carbapénème Microbiology (medical) carbapenemases Carbapenem resistance carbapenem resistance entérobactérie Microbial Sensitivity Tests MESH: Carbapenem-Resistant Enterobacteriaceae Sensitivity and Specificity Microbiology beta-Lactamases 03 medical and health sciences Bacterial Proteins Enterobacteriaceae Animals microbiologie selective agar Molecular Biology 030304 developmental biology Host Pathogen Interaction & Diagnostics Bacteriological Techniques 030306 microbiology Animal Bacteriology [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology Host Pathogen Interactie & Diagnostiek MESH: Sensitivity and Specificity Agar veterinary medicine Carbapenem-Resistant Enterobacteriaceae Bacteriologie Host Pathogen Interactie & Diagnostiek |
| Zdroj: | One Health EJP IMPART project, Dierikx, C, Börjesson, S, Perrin-Guyomard, A, Haenni, M, Norström, M, Divon, H H, Ilag, H K, Granier, S A, Hammerum, A, Kjeldgaard, J S, Pauly, N, Randall, L, Anjum, M F, Smialowska, A, Franco, A, Veldman, K & Slettemeås, J S 2022, ' A European multicenter evaluation study to investigate the performance on commercially available selective agar plates for the detection of carbapenemase producing Enterobacteriaceae ', Journal of Microbiological Methods, vol. 193, 106418 . https://doi.org/10.1016/j.mimet.2022.106418 Journal of Microbiological Methods 193 (2022) Journal of Microbiological Methods Journal of Microbiological Methods, 2022, 193, pp.106418. ⟨10.1016/j.mimet.2022.106418⟩ Journal of Microbiological Methods, 193 |
| ISSN: | 0167-7012 |
| Popis: | The European Food Safety Authority (EFSA) advised to prioritize monitoring carbapenemase producing Enterobacteriaceae (CPE) in food producing animals. Therefore, this study evaluated the performance of different commercially available selective agars for the detection of CPE using spiked pig caecal and turkey meat samples and the proposed EFSA cultivation protocol. Eleven laboratories from nine countries received eight samples (four caecal and four meat samples). For each matrix, three samples contained approximately 100 CFU/g CPE, and one sample lacked CPE. After overnight enrichment in buffered peptone water, broths were spread upon Brilliance™ CRE Agar (1), CHROMID® CARBA (2), CHROMagar™ mSuperCARBA™ (3), Chromatic™ CRE (4), CHROMID® OXA-48 (5) and Chromatic™ OXA-48 (6). From plates with suspected growth, one to three colonies were selected for species identification, confirmation of carbapenem resistance and detection of carbapenemase encoding genes, by methods available at participating laboratories. Of the eleven participating laboratories, seven reported species identification, susceptibility tests and genotyping on isolates from all selective agar plates. Agars 2, 4 and 5 performed best, with 100% sensitivity. For agar 3, a sensitivity of 96% was recorded, while agar 1 and 6 performed with 75% and 43% sensitivity, respectively. More background flora was noticed for turkey meat samples than pig caecal samples. Based on this limited set of samples, most commercially available agars performed adequately. The results indicate, however, that OXA-48-like and non-OXA-48-like producers perform very differently, and one should consider which CPE strains are of interest to culture when choosing agar type. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect