QTLs for Woolly Poplar Aphid (Phloeomyzus passerinii L.) Resistance Detected in an Inter-Specific Populus deltoides x P. nigra Mapping Population
| Autor: | Francesca Desiderio, Luigi Cattivelli, Alberto Gianinetti, Gianni Allegro, Giorgia Carletti, Lorenzo Vietto, Andrea Carra, Paolo Bagnaresi, Giampiero Valè, G. Nervo |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Genetic Linkage QTL lcsh:Medicine Plant Science Plant Genetics 01 natural sciences Trees Plant Genomics mapping lcsh:Science Aphid Disease Resistance education.field_of_study Multidisciplinary Chromosome Mapping food and beverages Genomics Plants Insects Populus poplar Poplars Research Article Biotechnology Genetic Markers Genotyping Arthropoda Quantitative Trait Loci Population Biology Quantitative trait locus Plant disease resistance Research and Analysis Methods resistance 03 medical and health sciences Species Specificity Gene mapping Genetic variation Botany Genetics Animals Plant breeding Molecular Biology Techniques Linkage Mapping education Molecular Biology Crosses Genetic Plant Diseases Analysis of Variance Base Sequence Gene Mapping fungi lcsh:R Organisms Biology and Life Sciences Molecular Sequence Annotation biology.organism_classification Invertebrates 030104 developmental biology Genetic Loci Genetic marker Aphids Plant Biotechnology lcsh:Q 010606 plant biology & botany |
| Zdroj: | PLoS ONE, Vol 11, Iss 3, p e0152569 (2016) PloS one 11 (2016): 1–18. doi:10.1371/journal.pone.0152569 info:cnr-pdr/source/autori:Carletti G., Carra A., Allegro G., Vietto L., Desiderio F., Bagnaresi P., Gianinetti A., Cattivelli L., Vale G., Nervo G./titolo:QTLs for Woolly Poplar aphid (Phloeomyzus passerinii L) resistance detected in an inter-specific Populus deltoides x P. nigra mapping population/doi:10.1371%2Fjournal.pone.0152569/rivista:PloS one/anno:2016/pagina_da:1/pagina_a:18/intervallo_pagine:1–18/volume:11 PLoS ONE |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0152569 |
| Popis: | The genus Populus represents one of the most economically important groups of forest trees. It is composed by approximately 30 species used for wood and non-wood products, phytoremediation and biomass. Poplar is subjected to several biological and environmental threats although, compared to annual crops, we know far less about the genetic bases of biotic stress resistance. Woolly poplar aphid (Phloeomyzus passerinii) is considered a main pest of cultivated poplars in European and American countries. In this work we present two high density linkage maps in poplar obtained by a genotyping by sequencing (GBS) approach and the identification of QTLs involved in Ph. passerinii resistance. A total of 5,667 polymorphic markers (5,606 SNPs and 61 SSRs) identified on expressed sequences have been used to genotype 131 plants of an F1 population P ×canadensis obtained by an interspecific mate between Populus deltoides (resistant to woolly poplar aphid) and Populus nigra (susceptible to woolly poplar aphid). The two linkage maps, obtained following the two-way pseudo-testcross mapping strategy, have been used to investigate the genetic bases of woolly poplar aphid resistance. One major QTL and two QTLs with minor effects (mapped on LGV, LGXVI and LG XIX) explaining the 65.8% of the genetic variance observed in the progeny in response to Ph. passerinii attack were found. The high density coverage of functional markers allowed the identification of three genes belonging to disease resistance pathway as putative candidates for P. deltoides resistance to woolly poplar aphid. This work is the first report on genetic of woolly poplar aphid genetic resistance and the resistant loci associated markers identified represent a valuable tool in resistance poplar breeding programs. |
| Databáze: | OpenAIRE |
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