Characterisation of a �-tubulin gene from the monogenean parasite, Gyrodactylus salaris Malmberg, 1957
| Autor: | C. M. Collins, C. O. Cunningham, K. A. Miller |
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| Rok vydání: | 2004 |
| Předmět: |
RNA Caps
Gyrodactylus DNA Complementary Sequence analysis Molecular Sequence Data Codon Initiator Sequence alignment macromolecular substances Biology Open Reading Frames Tubulin Complementary DNA Animals Parasite hosting Amino Acid Sequence RNA Messenger Gene Genes Helminth Genetics Base Sequence Sequence Homology Amino Acid General Veterinary Sequence Analysis DNA General Medicine DNA Helminth biology.organism_classification Open reading frame Infectious Diseases Insect Science Gyrodactylus salaris Codon Terminator Parasitology Trematoda RNA Helminth Sequence Alignment |
| Zdroj: | Parasitology Research. 92:390-399 |
| ISSN: | 1432-1955 0932-0113 |
| DOI: | 10.1007/s00436-003-1028-x |
| Popis: | The cDNA and two partial genomic sequences of beta-tubulin genes have been isolated from the monogenean parasite Gyrodactylus salaris. The cDNA sequence is not represented by either of the genomic sequences, implying that at least three isotypes of the gene exist in G. salaris. The sequences show regions of high homology with other helminth beta-tubulin genes. This represents the first isolation of a beta-tubulin gene from a monogenean and contributes to the overall characterisation of these genes within the helminths. This is an important area, as anthelmintic resistance is increasing against benzimidazole drugs that target the beta-tubulin gene. Benzimidazole drugs have been tested successfully against Gyrodactylus parasites, but their use is not widespread. Should it increase, analysis of the beta-tubulin gene may provide a tool for monitoring resistance development and improving management practises. Use of the beta-tubulin gene in the identification of Gyrodactylus species may prove complex due to the presence of different isotypes. |
| Databáze: | OpenAIRE |
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