Cocaine Reduces Ciliary Beat Frequency of Human Nasal Epithelial Cells
| Autor: | C Emika Mueller, Richard Birk, A Nastev, J. Ulrich Sommer, Benedikt Kramer, Daniel Haeussler, Boris A. Stuck, Wieland Behr, Angela Schell, Karl Hoermann |
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| Rok vydání: | 2020 |
| Předmět: |
Cancer Research
Mucous membrane of nose Cell Count Pharmacology General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 0302 clinical medicine Cocaine Medicine Humans Cocaine hydrochloride Cilia Ciliary beating business.industry Cilium Cartilage Healthy subjects Epithelial Cells In vitro Nasal Mucosa medicine.anatomical_structure 030220 oncology & carcinogenesis business Cocaine abuse Research Article |
| Zdroj: | In Vivo |
| ISSN: | 1791-7549 |
| Popis: | Background/aim Cocaine is a widely used recreational drug and is known for its nasal complications including epithelial, cartilage and bone damage. The aim of the study was to analyze the impact of cocaine on ciliary beat frequency (CBF) of human nasal epithelial cells and therefore better understand its side effects on nasal mucosa. Materials and methods Nasal epithelial cells of 21 healthy subjects were harvested and exposed in vitro to cocaine hydrochloride solutions ranging from 0.875% to 7%. High-speed video footage was acquired with phase contrast microscopy and CBF was analyzed with Sissons-Ammons Video Analysis (SAVA) software. Results All tested concentrations led to a significant reduction in CBF compared to the control. Effects increased over time and with concentration. A mechanical inhibition of cilia by cocaine crystals was also observed. Conclusion We assume that CBF reduction is part of the pathomechanism leading to nasal complications in cocaine abuse. Considering these results, clinical usage of cocaine should be critically evaluated and restricted to select cases only. |
| Databáze: | OpenAIRE |
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