Studies on Seryl and Phenyl-alanyl Synthetases from Ascitic Tumor Cells Treated with 20-Methylcholanthrene
| Autor: | Pedersen K, Kieler J, Qvist R |
|---|---|
| Rok vydání: | 1973 |
| Předmět: |
Phenylalanine
Tumor cells Aminoacylation Biology Cm sephadex Chromatography DEAE-Cellulose General Biochemistry Genetics and Molecular Biology Cell Line Amino Acyl-tRNA Synthetases Serine Mice Adenosine Triphosphate RNA Transfer Protein biosynthesis Animals Carbon Radioisotopes Amino Acids Chromatography 20-Methylcholanthrene Neoplasms Experimental Molecular biology Rats Kinetics Liver Biochemistry Transfer RNA Cancer cell Chromatography Gel Hydroxyapatites Methylcholanthrene |
| Zdroj: | Experimental Biology and Medicine. 144:714-721 |
| ISSN: | 1535-3699 1535-3702 |
| DOI: | 10.3181/00379727-144-37668 |
| Popis: | SummaryThe aminoacylation of tRNA is an important step in the regulation of protein synthesis. The aminoacyl-tRNA synthetases may have a regulatory function in the transformation of normal cells to neoplastic cells.Seryl and phenylalanyl synthetase have been isolated from ascitic tumor cells which were previously treated with 20-methyl-cholanthrene. The different steps of preparation are (NH4)2SO4 precipitation of the 105,000g supernatant (between 50 and 65% saturation), chromatography on DEAE cellulose, CM Sephadex C-50 and hydroxy-apatite.The purification fold of the seryl-tRNA synthetase was 450 times with the molecular weight of 120,000. The present studies have shown that the Km for serine was about 20 times higher in MCA-treated cells than in the controls, while the Km for ATP was only increased 3–4 times. These values indicate that the synthetases possibly have a regulatory role in the protein synthesis of the cancer cells with and without treatment with MCA. Similar differences were not found in t... |
| Databáze: | OpenAIRE |
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