MICAL2 enhances branched actin network disassembly by oxidizing Arp3B-containing Arp2/3 complexes
| Autor: | Christophe Guérin, Pavithra Singaravelu, Laurent Blanchoin, Naoko Kogata, Svend Kjaer, Davide Carra, Michael Way, Chiara Galloni, Jasmine V. Abella, David J. Barry |
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| Přispěvatelé: | The Francis Crick Institute, Cellular Signalling and Cytoskeletal Function Laboratory, The Francis Crick Institute, Structural Biology Science Technology Platform, CytoMorphoLab, Physiologie cellulaire et végétale (LPCV), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Grenoble Alpes (UGA)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Grenoble Alpes (UGA), CytoMorphoLab UMR976 HIPI, CEA, INSERM, Université de Paris, Paris, France, The Francis Crick Institute, Advanced Light Microscopy Facility, Imperial College, Department of infectious diseases, Cancer Research UK (FC001209), UK Medical Research Council (FC001209), Wellcome Trust (FC001209) funding at the Francis Crick Institute, European Project: Grant 810207, European Project: 741773,AAA, Martin-Laffon, Jacqueline, European Union’s Horizon 2020 research and innovation programme (grant 810207) - Grant 810207 - INCOMING, Adaptive Actin Architectures - AAA - 741773 - INCOMING, Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Gene isoform
[SDV.BC]Life Sciences [q-bio]/Cellular Biology macromolecular substances Biochemistry Article Actin-Related Protein 2-3 Complex 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Oxidizing agent Threonine [SDV.BC] Life Sciences [q-bio]/Cellular Biology Actin Cytoskeleton 030304 developmental biology 0303 health sciences Methionine biology Cell Biology Monooxygenase Actins Glutamine Actin Cytoskeleton chemistry biology.protein Biophysics 030217 neurology & neurosurgery Cortactin |
| Zdroj: | The Journal of Cell Biology Journal of Cell Biology Journal of Cell Biology, 2021, 220 (8), pp.e202102043. ⟨10.1083/jcb.202102043⟩ Journal of Cell Biology, Rockefeller University Press, 2021, 220 (8), pp.e202102043. ⟨10.1083/jcb.202102043⟩ |
| ISSN: | 1540-8140 0021-9525 |
| DOI: | 10.1083/jcb.202102043⟩ |
| Popis: | Galloni, Carra, et al. demonstrate that Arp3B isoform–specific Arp2/3 complexes generate branched actin networks with faster disassembly kinetics. This increased turnover is due to oxidation of Met293 of Arp3B by the methionine monooxygenase MICAL2, which is recruited to the branched actin network by coronin 1C. The mechanisms regulating the disassembly of branched actin networks formed by the Arp2/3 complex still remain to be fully elucidated. In addition, the impact of Arp3 isoforms on the properties of Arp2/3 are also unexplored. We now demonstrate that Arp3 and Arp3B isocomplexes promote actin assembly equally efficiently but generate branched actin networks with different disassembly rates. Arp3B dissociates significantly faster than Arp3 from the network, and its depletion increases actin stability. This difference is due to the oxidation of Arp3B, but not Arp3, by the methionine monooxygenase MICAL2, which is recruited to the actin network by coronin 1C. Substitution of Arp3B Met293 by threonine, the corresponding residue in Arp3, increases actin network stability. Conversely, replacing Arp3 Thr293 with glutamine to mimic Met oxidation promotes disassembly. The ability of MICAL2 to enhance network disassembly also depends on cortactin. Our observations demonstrate that coronin 1C, cortactin, and MICAL2 act together to promote disassembly of branched actin networks by oxidizing Arp3B-containing Arp2/3 complexes. |
| Databáze: | OpenAIRE |
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