Inhibition of nattokinase against the production of poly (γ-glutamic Acid) in Bacillus subtilis natto
| Autor: | Junliu Chen, Yipeng Zang, Li Wang, Ning Liu, Chenrui Yu, Mengmeng Wang, Kangjin Hong, Guangjun Nie |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Cell Glutamic Acid Bioengineering Bacillus subtilis (natto) 01 natural sciences Applied Microbiology and Biotechnology 03 medical and health sciences chemistry.chemical_compound Industrial Microbiology 010608 biotechnology Cell density Extracellular medicine Biomass Subtilisins Chemistry Substrate (chemistry) General Medicine Glutamic acid Culture Media 030104 developmental biology medicine.anatomical_structure Biochemistry Polyglutamic Acid Yield (chemistry) Fermentation Nattokinase Biotechnology Bacillus subtilis |
| Zdroj: | Biotechnology letters. 42(11) |
| ISSN: | 1573-6776 |
| Popis: | To study the effect of nattokinse (NK) on the synthesis of poly(γ-glutamic acid) (γ-PGA) in Bacillus subtilis natto. γ-PGA yield significantly decreased as NK was added in the original medium. With the increment of NK dosage, the yield decreased increasingly, but biomass increased instead of decreasing. The fact that cell density triggers the synthesis of γ-PGA is a controversial issue. γ-PGA yield and biomass closely correlate with addition time of NK. The later the addition of NK, the more γ-PGA yield decreased but the more biomass increased. It is concluded that cell hunger is a key factor to trigger the transmission of the cell density signal, and NK may inhibit γ-PGA synthesis by alleviating cell hunger. Besides, NK may reduce γ-PGA yield by degrading extracellular γ-PGA molecules. The study of adding L-glutamate of 0–20 g/L to the original medium showed that low concentration of L-glutamate (less than 5 g/L) could promote the synthesis of NK and γ-PGA, and thus NK may inhibit γ-PGA synthesis through strengthening substrate competition. NK mainly inhibits γ-PGA synthesis in Bacillus subtilis natto through alleviating cell starvation and strengthening substrate competition, and reduces γ-PGA yield through degrading extracellular γ-PGA molecules. |
| Databáze: | OpenAIRE |
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