Human–Ovine Comparative Sequencing of a 250-kb Imprinted Domain Encompassing the Callipyge (clpg) Locus and Identification of Six Imprinted Transcripts: DLK1, DAT, GTL2, PEG11, antiPEG11, and MEG8
| Autor: | Carole Charlier, Latifa Karim, Karin Segers, Gabor Gyapay, Jean Weissenbach, Michel Georges, Danny Wagenaar, Stéphane Berghmans, Noelle E. Cockett, T. L. Shay, Olivier Jaillon |
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| Rok vydání: | 2001 |
| Předmět: |
Genetic Markers
Guanine Letter Transcription Genetic Molecular Sequence Data Locus (genetics) Sequence alignment Biology Cytosine Genomic Imprinting Sequence Homology Nucleic Acid Genetics Animals Humans Muscle Skeletal Genetics (clinical) Repetitive Sequences Nucleic Acid Expressed Sequence Tags Base Composition Expressed sequence tag Sheep Intracellular Signaling Peptides and Proteins Computational Biology Membrane Proteins Proteins Sequence Analysis DNA Polar overdominance CpG site Genetic marker GenBank CpG Islands RNA Long Noncoding Genomic imprinting Sequence Alignment |
| Zdroj: | Genome Research. 11:850-862 |
| ISSN: | 1549-5469 1088-9051 |
| DOI: | 10.1101/gr.172701 |
| Popis: | Two ovine BAC clones and a connecting long-range PCR product, jointly spanning ∼250 kb and representing most of theMULGE5-OY3 marker interval known to contain the clpglocus, were completely sequenced. The resulting genomic sequence was aligned with its human ortholog and extensively annotated. Six transcripts, four of which were novel, were predicted to originate from within the analyzed region and their existence confirmed experimentally: DLK1, DAT, GTL2, PEG11, antiPEG11, and MEG8. RT-PCR experiments performed on a range of tissues sampled from an 8-wk-old animal demonstrated the preferential expression of all six transcripts in skeletal muscle, which suggests that they are under control of common regulatory elements. The six transcripts were also shown to be subject to parental imprinting: DLK1, DAT, andPEG11 were shown to be paternally expressed and GTL2,antiPEG11, and MEG8 to be maternally expressed.[The sequence data described in this paper have been submitted to the GenBank data library under accession no. AF354168.] |
| Databáze: | OpenAIRE |
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