Comparative Antimicrobial Activities of Aerosolized Sodium Hypochlorite, Chlorine Dioxide, and Electrochemically Activated Solutions Evaluated Using a Novel Standardized Assay
| Autor: | Darren M. Reynolds, Gareth M. Robinson, Robin Thorn |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Methicillin-Resistant Staphylococcus aureus
Biocide Sodium Hypochlorite Microorganism Colony Count Microbial Polypropylenes medicine.disease_cause Microbiology chemistry.chemical_compound medicine Bioassay Pharmacology (medical) Aerosolization Aerosols Spores Bacterial Pharmacology Analytical Procedures Chlorine dioxide Microbial Viability Humidity Oxides Electrochemical Techniques Stainless Steel Antimicrobial Solutions Infectious Diseases chemistry Staphylococcus aureus Sodium hypochlorite Pseudomonas aeruginosa Biological Assay Chlorine Compounds Bacillus subtilis Disinfectants Nuclear chemistry |
| Zdroj: | Antimicrobial Agents and Chemotherapy. 57:2216-2225 |
| ISSN: | 1098-6596 0066-4804 |
| Popis: | The main aim of this study was to develop a standardized experimental assay to enable differential antimicrobial comparisons of test biocidal aerosols. This study represents the first chlorine-matched comparative assessment of the antimicrobial activities of aerosolized sodium hypochlorite, chlorine dioxide, and electrochemically activated solution (ECAS) to determine their relative abilities to decontaminate various surface-associated health care-relevant microbial challenges. Standard microbiological challenges were developed by surface-associating typed Pseudomonas aeruginosa , Staphylococcus aureus , Bacillus subtilis spores, or a clinical methicillin-resistant S. aureus (MRSA) strain on stainless steel, polypropylene, or fabric. All test coupons were subjected to 20-min biocidal aerosols of chlorine-matched (100 ppm) sodium hypochlorite, chlorine dioxide, or ECAS within a standard aerosolization chamber using a commercial humidifier under defined conditions. Biocidal treatment type and material surface had a significant effect on the number of microorganisms recovered from various material surfaces following treatment exposure. Under the conditions of the assay, the order of antimicrobial efficacy of biocidal aerosol treatment was as follows: ECAS > chlorine dioxide > sodium hypochlorite. For all biocides, greater antimicrobial reductions were seen when treating stainless steel and fabric than when treating plastic-associated microorganisms. The experimental fogging system and assay protocol designed within this study were shown capable of differentiating the comparative efficacies of multiple chlorine-matched biocidal aerosols against a spectrum of target organisms on a range of test surface materials and would be appropriate for testing other biocidal aerosol treatments or material surfaces. |
| Databáze: | OpenAIRE |
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