Myotonic dystrophy protein kinase is expressed in embryonic myocytes and is required for myotube formation
| Autor: | Alicia F. Paulson, Erin B. Harmon, Michelle L. Harmon, M. Benjamin Perryman, Tricia D. Larsen |
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| Rok vydání: | 2008 |
| Předmět: |
medicine.medical_specialty
Recombinant Fusion Proteins Blotting Western Green Fluorescent Proteins Muscle Fibers Skeletal Apoptosis Chick Embryo Biology Protein Serine-Threonine Kinases Transfection Myotonic dystrophy Myotonin-Protein Kinase Cell Line Mice Internal medicine medicine Myocyte Animals Myocytes Cardiac Protein kinase A Myogenin Cells Cultured Muscle Cells Microscopy Confocal Myogenesis Cardiac myocyte Cell Differentiation medicine.disease Immunohistochemistry Cell biology Endocrinology Trinucleotide repeat expansion C2C12 Chickens Developmental Biology |
| Zdroj: | Developmental dynamics : an official publication of the American Association of Anatomists. 237(9) |
| ISSN: | 1058-8388 |
| Popis: | Myotonic dystrophy (DM1) is a multi-systemic disease caused by a triplet nucleotide repeat expansion in the 3′ untranslated region of the gene coding for myotonic dystrophy protein kinase (DMPK). The primary pathophysiology of DM1 is thought to result from RNA transport and processing defects. The function of DMPK in development or any potential role in DM1 remains unknown. Here we report a novel role for DMPK in myogenesis. We have discovered a specific expression pattern of DMPK in mouse and chick embryonic development. DMPK is expressed in postmitotic cardiac and skeletal myocytes and developmental signaling centers. During cardiac myocyte maturation, DMPK migrates from perinuclear to cellular membrane localization. Manipulating DMPK levels in cultured cardiac and skeletal myocytes has revealed a key role for DMPK in myocyte differentiation. Overexpression of DMPK induces cell rounding and apoptosis in myocytes. In addition, DMPK is necessary for myogenin expression in differentiating C2C12 myoblasts. Developmental Dynamics 237:2353–2366, 2008. © 2008 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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