Biomimetic, smart and multivalent ligands for G-quadruplex isolation and bioorthogonal imaging
| Autor: | Pauline Lejault, Joanna Zell, David Monchaud, Francesco Rota Sperti, Thibaut Charbonnier, Ibai E. Valverde, Claire Bernhard |
|---|---|
| Přispěvatelé: | Institut de Chimie Moléculaire de l'Université de Bourgogne [Dijon] (ICMUB), Centre National de la Recherche Scientifique (CNRS)-Université de Bourgogne (UB)-Institut de Chimie du CNRS (INC) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Cell Membrane Permeability [SDV]Life Sciences [q-bio] Chemical biology Computational biology Cell fate determination Ligands 010402 general chemistry G-quadruplex Biochemistry 01 natural sciences 03 medical and health sciences Biomimetic Materials Nucleic Acids Humans [CHIM]Chemical Sciences Biotinylation 030304 developmental biology 0303 health sciences Molecular Structure biology 010405 organic chemistry [CHIM.ORGA]Chemical Sciences/Organic chemistry Chemistry Optical Imaging General Medicine Small molecule 0104 chemical sciences G-Quadruplexes [SDV.BBM.BP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biophysics 030104 developmental biology MCF-7 Cells Click chemistry biology.protein Molecular Medicine Click Chemistry Bioorthogonal chemistry Avidin |
| Zdroj: | ACS Chemical Biology ACS Chemical Biology, American Chemical Society, 2021, 16 (5), pp.905-914. ⟨10.1021/acschembio.1c00111⟩ |
| ISSN: | 1554-8929 1554-8937 |
| DOI: | 10.1021/acschembio.1c00111⟩ |
| Popis: | G-quadruplexes (G4s) continue to gather wide attention in the field of chemical biology as their prevalence in the human genome and transcriptome strongly suggests that they may play key regulatory roles in cell biology. G4-specific, cell-permeable small molecules (G4-ligands) innovately permit the interrogation of cellular circuitries in order to assess to what extent G4s influence cell fate and functions. Here, we report on multivalent, biomimetic G4-ligands referred to as TASQs that enable both the isolation and visualization of G4s in human cells. Two biotinylated TASQs, BioTASQ and BioCyTASQ, are indeed efficient molecular tools to fish out G4s of mixtures of nucleic acids through simple affinity capture protocols and to image G4s in cells via a biotin/avidin pretargeted imaging system first applied here to G4s, found to be a reliable alternative to in situ click chemistry.Abstract Figure |
| Databáze: | OpenAIRE |
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