Analysis of a plasmid encoding botulinum neurotoxin type G gene in Clostridium argentinense
| Autor: | Keiji Oguma, Masakiyo Sakaguchi, Tomonori Suzuki, Yoshihiko Sakaguchi, Yumiko Yamamoto, Tomoko Kohda, Shunji Kozaki, Shunji Hayashi, Jumpei Uchiyama, Akira Také, Koji Hosomi, Kazuyoshi Gotoh, Masafumi Mukamoto |
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| Rok vydání: | 2020 |
| Předmět: |
DNA
Bacterial Botulinum Toxins Microbiology DNA sequencing Evolution Molecular 03 medical and health sciences Clostridium argentinense Plasmid RNA Ribosomal 16S Gene cluster medicine Botulism Gene Phylogeny 030304 developmental biology Clostridium 0303 health sciences biology Strain (chemistry) 030306 microbiology biology.organism_classification medicine.disease Molecular biology Botulinum neurotoxin Infectious Diseases Multigene Family Clostridium Infections Sequence Analysis Plasmids |
| Zdroj: | Anaerobe. 66:102281 |
| ISSN: | 1075-9964 |
| DOI: | 10.1016/j.anaerobe.2020.102281 |
| Popis: | Clostridium argentinense produces botulinum neurotoxin type G (BoNT/G). We sequenced and analyzed the plasmid harboring the bont/G gene, designated pCAG, in C. argentinense strain 2740. The pCAG consisted of 140,070 bp containing the bont/G gene cluster. Although this gene cluster showed high similarities in its DNA sequence and ORF arrangement to those of other bont gene clusters, the other regions of the plasmid did not. A phylogenetic study suggested that pCAG had a unique evolutionary history compared with other clostridial bont-harboring plasmids. This suggests that pCAG is possibly a novel type of plasmid expressing the bont/G gene in C. argentinense. |
| Databáze: | OpenAIRE |
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