Variability of ACOX1 Gene Polymorphisms across Different Horse Breeds with Regard to Selection Pressure
| Autor: | Adrianna D Musiał, Katarzyna Ropka-Molik, Monika Stefaniuk-Szmukier, Katarzyna Piórkowska, Grzegorz Myćka |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Polish Konik Biology Arabian horses 03 medical and health sciences 0302 clinical medicine peroxisomal acyl-coenzyme A oxidase 1 lcsh:Zoology Genotype SNP lcsh:QL1-991 Allele draft horses Hucul chemistry.chemical_classification Genetics lcsh:Veterinary medicine General Veterinary Fatty acid Horse Thoroughbred 030104 developmental biology chemistry Genetic marker lcsh:SF600-1100 ACOX1 Animal Science and Zoology lipids (amino acids peptides and proteins) genetic marker Restriction fragment length polymorphism 030217 neurology & neurosurgery |
| Zdroj: | Animals Volume 10 Issue 12 Animals, Vol 10, Iss 2225, p 2225 (2020) |
| ISSN: | 2076-2615 |
| DOI: | 10.3390/ani10122225 |
| Popis: | The ACOX1 gene encodes peroxisomal acyl-coenzyme A oxidase 1, the first enzyme in the fatty acid &beta oxidation pathway, which could be significant for organisms exposed to long periods of starvation and harsh living conditions. We hypothesized that variations within ACOX1, revealed by RNA Sequencing (RNA-Seq), might be based on adaptation to living conditions and had resulted from selection pressure. There were five different horse breeds used in this study, representing various utility types: Arabian, Thoroughbred, Polish Konik, draft horses, and Hucul. The single-nucleotide polymorphism (SNP) located in the ACOX1 (rs782885985) was used as a marker and was identified using the PCR restriction fragment length polymorphism method (PCR-RFLP). Results indicated extremely different genotype and allele distributions of the ACOX1 gene across breeds. A predominance of the G allele was exhibited in horses that had adapted to difficult environmental conditions, namely, Polish Konik and Huculs, which are considered to be primitive breeds. The prevalence of the T allele in Thoroughbreds indicated that ACOX1 is significant in energy metabolism during flat racing. |
| Databáze: | OpenAIRE |
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