Distribution of infectious bronchitis virus strains in different organs and evidence of vertical transmission in natural infection

Autor: Juliana Lopes Rangel Fietto, Abelardo Silva Júnior, Gustavo Costa Bressan, Giuliana Loreto Saraiva, Claiton Gonçalves Pereira, Márcia Rogéria de Almeida, Maria Aparecida Scatamburlo Moreira, Pedro Marcus Pereira Vidigal
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0301 basic medicine
medicine.medical_specialty
animal structures
Infectious bronchitis virus
Biology
Infectious bronchitis virus strain
Virus
03 medical and health sciences
Medical microbiology
Virology
medicine
Animals
Bursa of Fabricius
Poultry Diseases
Infectious bronchitis virus infection
Transmission (medicine)
Cecal tonsil
Infectious Bronchitis Virus Strain
Animal Structures
General Medicine
Sequence Analysis
DNA
Avian infectious bronchitis
biology.organism_classification
Vertical Transmission
Cecal Tonsil
Small intestine
Infectious Disease Transmission
Vertical
030104 developmental biology
medicine.anatomical_structure
embryonic structures
Spike Glycoprotein
Coronavirus
Oviduct
Vertical transmission
Original Article
Infectious Bronchitis Virus Infection
Coronavirus Infections
Chickens
Zdroj: Archives of Virology
LOCUS Repositório Institucional da UFV
Universidade Federal de Viçosa (UFV)
instacron:UFV
ISSN: 1432-8798
0304-8608
Popis: On the basis of partial sequencing of the infectious bronchitis virus (IBV) S1 gene, this study investigated the molecular diversity of the virus in two life periods of a batch of breeding hens at the field level. The chicks were vaccinated against IBV on the second day of life with the vaccine Ma5, but at the age of 18 days, they exhibited clinical signs and macroscopic lesions compatible with avian infectious bronchitis (IB). In the clinical disease stage, the Ma5 vaccine strain was detected in the trachea, lungs, and small intestine of the chicks, while IBV variants were detected in the bursa of Fabricius and kidneys. Subsequently, new samples were collected from the same batch at the end of the production cycle. In this phase, the Ma5 vaccine strain was detected in the kidneys, small intestine, and oviduct of the hens. However, a previously unidentified IBV variant was found in the cecal tonsils. Additionally, a fragment of viral RNA with that was completely identical to the corresponding region of the Ma5 vaccine was detected in the allantoic fluid of viable embryos from the hens under study after 18 days of incubation. These findings suggest that, in addition to the Ma5 vaccine, other strains of IBV variants can coexist, seeming to establish a chronic infection in the chickens, and that they can potentially be transmitted vertically. These results may assist in immunoprophylaxis control programs against IBV. Electronic supplementary material The online version of this article (doi:10.1007/s00705-016-3030-5) contains supplementary material, which is available to authorized users.
Databáze: OpenAIRE
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