Genetic variability of the activity of bidirectional promoters : a pilot study in bovine muscle
| Autor: | Abderrahman Maftah, Emmanuelle Rebours, Rabia Letaief, Gabriel Guillocheau, Véronique Léjard, Diane Esquerre, Christy J. Vander Jagt, Mekki Boussaha, Dominique Rocha, Gilles Renand, Anis Djari, Amanda J. Chamberlain, Cédric Meersseman, Daniel Petit, Christophe Klopp |
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| Přispěvatelé: | Génétique Animale et Biologie Intégrative (GABI), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Unité de Génétique Moléculaire Animale (UGMA), Université de Limoges (UNILIM)-Institut National de la Recherche Agronomique (INRA), Génétique Physiologie et Systèmes d'Elevage (GenPhySE ), École nationale supérieure agronomique de Toulouse [ENSAT]-Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Unité de Mathématiques et Informatique Appliquées de Toulouse (MIAT INRA), Institut National de la Recherche Agronomique (INRA), Dairy Futures Cooperat Res, Ctr, Bundoora, AgriBio, Dept Econ Dev Jobs Transport & Resources, Bundoora, Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-École nationale supérieure agronomique de Toulouse [ENSAT], AgroParisTech-Institut National de la Recherche Agronomique (INRA), Unité de Génétique Moléculaire Animale (UMR GMA) |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine muscle [SDV]Life Sciences [q-bio] Pilot Projects Single-nucleotide polymorphism Biology Polymorphism Single Nucleotide 03 medical and health sciences cattle genome Genetic variation genetic variability Genetics Animals Genetic variability Promoter Regions Genetic bidirectional promoter Molecular Biology Gene Regulation of gene expression Back Muscles Gene Expression Profiling Proteins Promoter General Medicine Full Papers Gene expression profiling Editor's Choice 030104 developmental biology Gene Expression Regulation Regulatory sequence Cattle |
| Zdroj: | DNA Research DNA Research, Oxford University Press (OUP), 2017, 24 (3), pp.221-233. ⟨10.1093/dnares/dsx004⟩ DNA Research 3 (24), 221-233. (2017) DNA Research: An International Journal for Rapid Publication of Reports on Genes and Genomes |
| ISSN: | 1340-2838 |
| Popis: | Bidirectional promoters are regulatory regions co-regulating the expression of two neighbouring genes organized in a head-to-head orientation. In recent years, these regulatory regions have been studied in many organisms; however, no investigation to date has been done to analyse the genetic variation of the activity of this type of promoter regions. In our study, we conducted an investigation to first identify bidirectional promoters sharing genes expressed in bovine Longissimus thoracis and then to find genetic variants affecting the activity of some of these bidirectional promoters. Combining bovine gene information and expression data obtained using RNA-Seq, we identified 120 putative bidirectional promoters active in bovine muscle. We experimentally validated in vitro 16 of these bidirectional promoters. Finally, using gene expression and whole-genome genotyping data, we explored the variability of the activity in muscle of the identified bidirectional promoters and discovered genetic variants affecting their activity. We found that the expression level of 77 genes is correlated with the activity of 12 bidirectional promoters. We also identified 57 single nucleotide polymorphisms associated with the activity of 5 bidirectional promoters. To our knowledge, our study is the first analysis in any species of the genetic variability of the activity of bidirectional promoters. |
| Databáze: | OpenAIRE |
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