Extent and Kinetics of Genetic Change in the omp1 Gene of Chlamydia trachomatis in Two Villages with Endemic Trachoma
| Autor: | Suphat Pecharatana, David Mabey, Lyn J. Hayes, Timothy J. Hampton, Peter J. Watt, Mark A. Pickett, Michael E. Ward, Robin L. Bailey |
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| Rok vydání: | 1995 |
| Předmět: |
Molecular Sequence Data
Porins Chlamydia trachomatis Locus (genetics) urologic and male genital diseases medicine.disease_cause Polymerase Chain Reaction law.invention law medicine Humans Immunology and Allergy Chlamydiaceae Amino Acid Sequence Gene Polymerase chain reaction DNA Primers Trachoma Base Sequence Sequence Homology Amino Acid biology Genetic Variation Antigenic shift medicine.disease biology.organism_classification Virology Kinetics Cross-Sectional Studies Infectious Diseases Genes Bacterial Chlamydiales Gambia Conjunctiva Bacterial Outer Membrane Proteins |
| Zdroj: | Europe PubMed Central |
| ISSN: | 1537-6613 0022-1899 |
| DOI: | 10.1093/infdis/172.1.268 |
| Popis: | Variants of Chlamydia trachomatis in two Gambian villages with hyperendemic trachoma were analyzed by omp1-based polymerase chain reaction and sequencing from conjunctival swabs. Samples collected over a 22-month period included a complete cross-sectional study of each village. Overall, 4 genovar A and 4 B variants were characterized by point mutations in the omp1 gene, resulting in changes in the inferred amino acid sequence. Two genovar A and 2 B variants accounted for 87% of the total ocular chlamydial infection in both villages. Although some flux in the prevalence of individual variants was observed over time, their overall distribution remained remarkably stable. There was no evidence of major antigenic shift arising from recombination events at the omp1 locus as described for genital tract infection. These results indicate that omp1 variation in these two trachoma-hyperendemic communities is limited and unlikely to hamper development of trachoma vaccines based on the major outer membrane protein. |
| Databáze: | OpenAIRE |
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