A Multiperspective Approach to Solvent Regulation of Enzymatic Activity : HMG-CoA Reductase
| Autor: | Simon Ebbinghaus, Oktavian Krenczyk, Axel Rosenhahn, Andreas von Gundlach, Martin A. Schroer, Frank Schulz, Christoph Rumancev, Christian Herrmann, Javier Iglesias-Fernández, Michael Dirkmann, Pandian Sokkar, Tobias Vöpel, Elsa Sanchez-Garcia, Victor Muñoz, Julia Nowack |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Acetonitriles Molecular model Chemie Ionic Liquids Calorimetry Molecular Dynamics Simulation Reductase 010402 general chemistry 01 natural sciences Biochemistry Active center 03 medical and health sciences Molecular dynamics X-Ray Diffraction Scattering Small Angle Molecular Biology chemistry.chemical_classification biology Organic Chemistry Enzyme assay 0104 chemical sciences Solvent Kinetics 030104 developmental biology Enzyme chemistry Alcohols Solvents Sulfolobus solfataricus Biophysics biology.protein Molecular Medicine Acyl Coenzyme A Solvent effects Biologie |
| Popis: | 3-Hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase was investigated in different organic cosolvents by means of kinetic and calorimetric measurements, molecular dynamics simulations, and small-angle X-ray scattering. The combined experimental and theoretical techniques were essential to complement each other's limitations in the investigation of the complex interaction pattern between the enzyme, different solvent types, and concentrations. In this way, the underlying mechanisms for the loss of enzyme activity in different water-miscible solvents could be elucidated. These include direct inhibitory effects onto the active center and structural distortions. |
| Databáze: | OpenAIRE |
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