Sodium butyrate modulates gut microbiota and immune response in colorectal cancer liver metastatic mice
| Autor: | Qinghua Dong, Hongyan Diao, Xujun Zhang, Ye Feng, Yiyang Hong, Guanyu Wang, Ximei Ma, Zhuha Zhou, Mengjing Fan |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Health Toxicology and Mutagenesis Gut flora Toxicology Metastasis 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Immune system Immunity Cell Line Tumor medicine Animals Mice Inbred BALB C biology Liver Neoplasms Cancer Sodium butyrate Cell Biology biology.organism_classification medicine.disease Natural killer T cell Gastrointestinal Microbiome 030104 developmental biology chemistry 030220 oncology & carcinogenesis Cancer research Butyric Acid Colorectal Neoplasms Dysbiosis |
| Zdroj: | Cell Biology and Toxicology. 36:509-515 |
| ISSN: | 1573-6822 0742-2091 |
| Popis: | Colorectal cancer (CRC) liver metastasis (CLM) is the leading death cause of CRC patients, but there is no satisfied approach to treat CLM. Gut microbiota plays a pivotal role in CRC initiation and development. Targeting dysbiosis of the gut microbiota might open up new opportunities for CLM treatment. Here, we investigated the efficacy of sodium butyrate (NaB), a major product of gut microbial fermentation, in modulating gut microbiota in CLM mice. NaB supplement decreased mouse colon cancer CT26 cell liver metastasis in intrasplenic tumor injection model of BALB/c mice. Using 16S rRNA gene sequencing, we found altered microbiota composition in CLM mice, characterized by increases of Firmicutes and Proteobacteria. NaB beneficially changed dysbiosis in CLM mice. Functional analysis of the KEGG pathways showed that NaB changed pathways related to immune system diseases and primary immunodeficiency in CLM mice. In addition, NaB decreased T regulatory cells and increased natural killer T cells and T helper 17 cells, accordingly decreased IL-10 and increased IL-17 secretion in CLM mice liver. In conclusion, NaB beneficially modulated gut microbiota and improved host immune response in CLM mice. These findings demonstrate the therapeutic potential of NaB in CLM treatment. |
| Databáze: | OpenAIRE |
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