Carbohydrate plasma expanders for passive tumor targeting: In vitro and in vivo studies
| Autor: | Karsten Mäder, Patrick Kreideweiß, Henrike Caysa, Stefan Hoffmann, Anja Leimert, Judith Kuntsche, Thomas Mueller |
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| Rok vydání: | 2013 |
| Předmět: |
Male
Tumor accumulation Biodistribution Hydroxyethyl starch Polymers and Plastics Cell Survival Plasma Substitutes Mice Nude Conjugated system Hydroxyethyl Starch Derivatives Mice chemistry.chemical_compound In vivo Materials Chemistry medicine Animals Humans Tissue Distribution Cytotoxicity Cells Cultured Passive tumor targeting Multispectral optical imaging Organic Chemistry HES Dextrans Hep G2 Cells Xenograft Model Antitumor Assays In vitro Tumor Burden Dextran chemistry Biochemistry Colonic Neoplasms Leukocytes Mononuclear Cytokines Female Cytokine induction medicine.drug Conjugate |
| Zdroj: | Hoffmann, S, Caysa, H, Kuntsche, J, Kreideweiss, P, Leimert, A, Müller, T & Mäder, K 2013, ' Carbohydrate plasma expanders for passive tumor targeting : In vitro and in vivo studies ', Carbohydrate Polymers, vol. 95, pp. 404-413 . https://doi.org/10.1016/j.carbpol.2013.03.033 |
| ISSN: | 0144-8617 |
| DOI: | 10.1016/j.carbpol.2013.03.033 |
| Popis: | The objective of this study was to investigate the suitability of carbohydrate plasma volume expanders asa novel polymer platform for tumor targeting. Many synthetic polymers have already been synthesized fortargeted tumor therapy, but potential advantages of these carbohydrates include inexpensive synthesis,constant availability, a good safety profile, biodegradability and the long clinical use as plasma expanders.Three polymers have been tested for cytotoxicity and cytokine activation in cell cultures and conjugatedwith a near-infrared fluorescent dye: hydroxyethyl starches (HES 200 kDa and HES 450 kDa) and dextran(DEX 500 kDa). Particle size and molecular weight distribution were determined by asymmetric flowfield-flow fractionation (AF4). The biodistribution was investigated non-invasively in nude mice usingmultispectral optical imaging. The most promising polymer conjugate was characterized in human coloncarcinoma xenograft bearing nude mice. A tumor specific accumulation of HES 450 was observed, whichproves it’s potential as carrier for passive tumor targeting. |
| Databáze: | OpenAIRE |
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