Mismatch-sensitive hybridization detection by peptide nucleic acids immobilized on a quartz crystal microbalance
| Autor: | João Roberto Fernandes, Xiaohua Cai, Mehmet Ozsoz, Peter E. Nielsen, Joseph Wang, Douglas H. Grant, and Asher Beglieter, Michael Mowat, Mian Jiang |
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| Rok vydání: | 1998 |
| Předmět: |
Peptide nucleic acid
Oligonucleotide Chemistry Nucleic Acid Heteroduplexes Nucleic Acid Hybridization Quartz crystal microbalance Biosensing Techniques Genes p53 Analytical Chemistry Nucleic acid thermodynamics chemistry.chemical_compound Biochemistry Nucleic acid Biophysics Molecular probe Oligonucleotide Probes Peptides Biosensor |
| Zdroj: | Analytical chemistry. 69(24) |
| ISSN: | 0003-2700 |
| Popis: | A quartz crystal microbalance DNA hybridization biosensor, based on thiol-derivatized peptide nucleic acid (PNA) probes, offers unusual in situ differentiation of single-base mismatches. A large excess of a single-base mismatch oligonucleotide has no effect on the frequency response of the target. Such remarkable distinction between perfect matches and mismatches is illustrated by the detection of a common mutation in the p53 gene. The greater specificity of the new mass-sensitive indicatorless hybridization device over those of analogous PNA-based carbon electrodes is attributed to the formation of a PNA monolayer and the use of a hydrophilic ethylene glycol linker. The improved specificity is coupled to very fast (3−5 min) hybridization in a low-ionic-strength medium. |
| Databáze: | OpenAIRE |
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