Cucurbitacin I induces apoptosis in ovarian cancer cells through oxidative stress and the p190B‑Rac1 signaling axis

Autor:	Xinjing Lin, Bin Han, Honglin Xu, Ming Yang, Ruli Li, Fu Liu, Jianbo Xiao, Sufan Tang
Rok vydání:	2020
Předmět:	rac1 GTP-Binding Protein 0301 basic medicine Cancer Research Programmed cell death Time Factors Cell Survival Cell medicine.disease_cause Biochemistry 03 medical and health sciences 0302 clinical medicine Cell Line Tumor Genetics medicine Humans Molecular Biology Ovarian Neoplasms Oncogene Cucurbitacin Chemistry GTPase-Activating Proteins Cell cycle medicine.disease Triterpenes Gene Expression Regulation Neoplastic Oxidative Stress 030104 developmental biology medicine.anatomical_structure Oncology Apoptosis 030220 oncology & carcinogenesis Cancer research Molecular Medicine Female Ovarian cancer Oxidative stress Signal Transduction
Zdroj:	Molecular Medicine Reports. 22:2545-2550
ISSN:	1791-3004 1791-2997
DOI:	10.3892/mmr.2020.11327
Popis:	Ovarian cancer is a serious threat to women's life and health, with a high mortality rate. Therefore, in addition to improving surgery for ovarian cancer, it is particularly important to develop novel drug treatments. In the present study, the anticancer effects of cucurbitacin I, a natural product, were investigated. Cucurbitacin I impaired the viability of SKVO3 cells in a concentration‑ and time‑dependent manner. Apoptosis was involved in the process of cucurbitacin I‑induced cell death, with an increase observed in cleaved‑caspase 3 and BAX, and a decrease in Bcl‑2. Cucurbitacin I caused a notable increase in intracellular reactive oxygen species, and regulated Kelch‑like ECH‑associated protein 1 and nuclear factor erythroid‑derived 2‑like 2 to decrease the expression of antioxidant‑related genes. In addition, Cucurbitacin I induced cell shrinkage by regulating the p190BRhoGAP (p190B)‑Rac1 signaling axis related to the cytoskeleton. In brief, these results suggested that cucurbitacin I induced cell death through oxidative stress and the p190B‑Rac1 signaling axis in SKVO3 cells. The results may provide novel evidence for the treatment of ovarian cancer.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::99bf6e151eed973493aa5e7310f22fba https://doi.org/10.3892/mmr.2020.11327 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
Nepřihlášeným uživatelům se plný text nezobrazuje	K zobrazení výsledku je třeba se přihlásit.