A new poly(I:C)-decorated PLGA-PEG nanoparticle promotes Mycobacterium tuberculosis fusion protein to induce comprehensive immune responses in mice intranasally
| Autor: | Pu He, Xiufen Du, Jiying Tan, Kun Xu, Tao Xie, Daquan Tan, Zongjie Hou, Wei Lv, Jiangyuan Han, Yang Gong, Yifan Zhang, Bingdong Zhu |
|---|---|
| Rok vydání: | 2022 |
| Předmět: |
Protein subunit
medicine.medical_treatment Microbiology Mycobacterium tuberculosis Mice Immune system Adjuvants Immunologic Immunity medicine Animals Adjuvants Vaccine Antigens Bacterial biology Chemistry Immunogenicity biology.organism_classification Fusion protein Molecular biology Immunity Humoral Mice Inbred C57BL Infectious Diseases Nanoparticles Female Nasal administration Adjuvant |
| Zdroj: | Microbial Pathogenesis. 162:105335 |
| ISSN: | 0882-4010 |
| DOI: | 10.1016/j.micpath.2021.105335 |
| Popis: | Protein-based subunit vaccine against tuberculosis (TB) is regarded as safer but with lower immunogenicity. To investigate effective adjuvant to improve the immunogenicity of TB subunit vaccine, we modified ploy(I:C) onto PLGA-PEG copolymer nanoparticle with polydopamine to produce a new nanoparticle adjuvant named “PLGA-PEG-poly(I:C)” (NP). M. tuberculosis fusion proteins Mtb10.4-HspX and ESAT-6-Rv2626c (M4) were encapsulated in the nanoparticles to produce the NP/M4 subunit vaccine. The PLGA-PEG/M4 nanoparticle was 200.21 ± 1.07 nm in diameter, and the polydispersity index (PDI) was 0.127 ± 0.02. Following modification with poly(I:C) by polydopamine, the NP/M4 was administered to C57BL/6 female mice intranasally and the immune responses were evaluated. The NP/M4 significantly induced antigen-specific CD4+ T cells proliferation, IL-2 and IFN-γ production. In addition, the NP/M4 could promote the production of antigen-specific IgG, IgG1, IgG2c in serum, and sIgA in lung washings. Overall, our results indicated that the NP would be a potential TB subunit vaccine adjuvant with the ability to induce strong Th1-type cell-mediated immunity and humoral immune responses. |
| Databáze: | OpenAIRE |
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