Selection of relatively exact reference genes for gene expression studies in goosegrass (Eleusine indica) under herbicide stress
| Autor: | Yan Liu, Cuilan Jiang, Hongjuan Huang, Jingchao Chen, Jie Zhang, Shouhui Wei, Chaoxian Zhang, Zhaofeng Huang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Glycine Eleusine indica Article Eleusine 03 medical and health sciences chemistry.chemical_compound Chloroplast Proteins Gene Expression Regulation Plant Stress Physiological Reference genes Gene expression Selection Genetic Gene Genetics Acetolactate synthase Multidisciplinary biology business.industry Herbicides biology.organism_classification Biotechnology Acetolactate Synthase 030104 developmental biology chemistry Glufosinate Glyphosate biology.protein Weed business Herbicide Resistance |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep46494 |
| Popis: | Goosegrass (Eleusine indica) is one of the most serious annual grassy weeds worldwide, and its evolved herbicide-resistant populations are more difficult to control. Quantitative real-time PCR (qPCR) is a common technique for investigating the resistance mechanism; however, there is as yet no report on the systematic selection of stable reference genes for goosegrass. This study proposed to test the expression stability of 9 candidate reference genes in goosegrass in different tissues and developmental stages and under stress from three types of herbicide. The results show that for different developmental stages and organs (control), eukaryotic initiation factor 4 A (eIF-4) is the most stable reference gene. Chloroplast acetolactate synthase (ALS) is the most stable reference gene under glyphosate stress. Under glufosinate stress, eIF-4 is the best reference gene. Ubiquitin-conjugating enzyme (UCE) is the most stable reference gene under quizalofop-p-ethyl stress. The gene eIF-4 is the recommended reference gene for goosegrass under the stress of all three herbicides. Moreover, pairwise analysis showed that seven reference genes were sufficient to normalize the gene expression data under three herbicides treatment. This study provides a list of reliable reference genes for transcript normalization in goosegrass, which will facilitate resistance mechanism studies in this weed species. |
| Databáze: | OpenAIRE |
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