Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD
| Autor: | A.C. Cottorello, Romário Cerqueira Leite, G.A. Carvalho-Castro, Henrique César Pereira Figueiredo, Carlos Augusto Gomes Leal |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Veterinary Medicine
Pathology medicine.medical_specialty clinical samples Short Communication lcsh:QR1-502 Computational biology Biology Real-Time Polymerase Chain Reaction sensitivity Sensitivity and Specificity Microbiology lcsh:Microbiology Shrimp Real-time polymerase chain reaction WSSV Molecular Diagnostic Techniques Penaeidae TaqMan medicine Animals Sensitivity (control systems) WSD real-time PCR |
| Zdroj: | Brazilian Journal of Microbiology, Volume: 44, Issue: 3, Pages: 901-904, Published: 15 NOV 2013 Brazilian Journal of Microbiology, Vol 44, Iss 3, Pp 901-904 (2013) Brazilian Journal of Microbiology v.44 n.3 2013 Brazilian Journal of Microbiology Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
| Popis: | The aims of this study were to standard and optimize a qPCR protocol with FAM-BHQ1 probe, and to compare its sensitivity against TaqMan qPCR and PCR methods to diagnose shrimp WSD. The FAM-BHQ1 qPCR presented higher clinical sensitivity and showed to be a robust alternative to detect WSSV in clinical samples. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|