Liarozole Inhibits Human Epidermal Retinoic Acid 4-Hydroxylase Activity and Differentially Augments Human Skin Responses to Retinoic Acid and Retinol In Vivo
| Autor: | Kwang J. Kim, Elizabeth A. Duell, John J. Voorhees, Sewon Kang |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
retinoids
P-450 Erythema medicine.drug_class Retinoic acid Retinoic acid 4-hydroxylase activity Tretinoin Human skin Dermatology Biochemistry chemistry.chemical_compound Cytochrome P-450 Enzyme System medicine Cytochrome P-450 Enzyme Inhibitors Humans Liarozole Retinoid Vitamin A Molecular Biology Skin irritation Dose-Response Relationship Drug integumentary system Imidazoles Retinol Androgen Antagonists Drug Synergism Cell Biology Retinoic Acid 4-Hydroxylase Molecular biology chemistry Epidermis medicine.symptom metabolism medicine.drug |
| Zdroj: | Journal of Investigative Dermatology. 107:183-187 |
| ISSN: | 0022-202X |
| Popis: | Metabolic inactivation of all-trans retinoic acid to 4-hydroxy retinoic acid occurs via a cytochrome P-450 enzyme. We investigated the effects of liarozole on the retinoic acid 4-hydroxylase activity of human epidermis and its ability to modify in vivo human skin responses to retinoic acid and all-trans retinol. Retinoic acid 4-hydroxylase activity induced in vivo by 4 d treatment with retinoic acid (0.1%) was inhibited in vitro by liarozole in a concentration-dependent manner. Comparable micromolar concentrations of liarozole were extracted from stratum corneum-free epidermis treated with 3% liarozole. Retinoic acid levels in liarozole-treated skin increased to 19 +/- 5 ng/g wet wt (mean +/- SEM, p < 0.002, n = 17) at 18 h and to 6 +/- 2 ng/g wet wt (p = 0.38, n = 17) at 48 h as compared to vehicle (not detectable). At 48 h, retinoic acid 4-hydroxylase activity was induced 9-fold over vehicle (p < 0.03, n = 8). At 96 h, no significant erythema or increased epidermal thickness was found when either retinoic acid (0.001%), all-trans retinol (0.0250%), or liarozole (3%) was applied individually, but when 0.001% retinoic acid and 3% liarozole were applied together, both erythema and increased epidermal thickness occurred. In contrast, 0.025% all-trans retinol and 3% liarozole together caused increased epidermal thickness but no erythema. These data demonstrate that, at doses used here, liarozole, although an effective inhibitor of retinoic acid 4-hydroxylase, cannot function alone like a retinoid in vivo, probably because of retinoic acid 4-hydroxylase induction. In the presence of a low dose retinoic acid or all-trans retinol, however, liarozole can amplify human skin responses to each retinoid in a manner characteristic of the retinoid at a higher dose (erythema and hyperplasia with retinoic acid; no erythema but hyperplasia with all-trans retinol). |
| Databáze: | OpenAIRE |
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