Phenolic and flavonoid contents, antioxidant and antimicrobial activities of leaf extracts from ten Algerian Ficus carica L. varieties
| Autor: | Mustapha Khali, Imen Baiti, Abderahim Benkhaled, Souhila Mahmoudi, Karima Benamirouche |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Antioxidant
lcsh:Arctic medicine. Tropical medicine lcsh:RC955-962 medicine.medical_treatment Flavonoid Bacillus cereus Ficus Antimicrobial activity 01 natural sciences Biochemistry Genetics and Molecular Biology (miscellaneous) chemistry.chemical_compound 0404 agricultural biotechnology Antioxidant activity Botany medicine Gallic acid Food science lcsh:QH301-705.5 chemistry.chemical_classification Flavonoids biology Chemistry 010401 analytical chemistry 04 agricultural and veterinary sciences Fig leaves biology.organism_classification Antimicrobial 040401 food science 0104 chemical sciences lcsh:Biology (General) Carica Phenolics Quercetin |
| Zdroj: | Asian Pacific Journal of Tropical Biomedicine, Vol 6, Iss 3, Pp 239-245 (2016) |
| ISSN: | 2221-1691 |
| Popis: | Objective To determine the total phenolic and flavonoid contents, antioxidant and antimicrobial activities of methanolic leaf extracts of ten Algerian fig ( Ficus carica L.) varieties (uniferous, biferous and caprifig tree). Methods Phenolics were extracted by Soxhlet method and analyzed by the Folin–Ciocalteu colorimetric method. Flavonoids were determined by aluminum trichloride assay and the antioxidant capacity was determined by the 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay. The antimicrobial activity was studied with the disc diffusion method and a macrodilution broth method was used to determine the minimal inhibitory concentrations and minimal lethal concentrations. Results The mean extract yield was 14.10% ± 0.66% ( n = 10). Leaf extract of biferous followed by uniferous varieties had the highest total phenolic contents [(52.296 ± 5.232) and (48.973 ± 2.015) mg gallic acid equivalent/g of dry plant extract respectively], flavonoids [(14.388 ± 0.333) and (14.136 ± 1.082) mg quercetin equivalent/g of dry plant extract] and antioxidant capacity [IC 50 (798.754 ± 108.590) and (825.004 ± 110.835) μg/mL]. Antioxidant capacity of fig leaves was significantly correlated with phenolic contents ( r = 0.748). These extracts showed bactericidal activity and moderate antifungal activity, and the minimal inhibitory concentrations and minimal lethal concentrations were determined on Bacillus cereus and Staphylococcus aureus . Conclusions All tested extracts contain phenolic compounds and exhibited an antioxidant activity and an antimicrobial effect against Gram-positive and Gram-negative bacteria. Further researches on identification and purification of phenolic compounds are required. |
| Databáze: | OpenAIRE |
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