Global clonal spread of mcr-3-carrying MDR ST34 Salmonella enterica serotype Typhimurium and monophasic 1,4,[5],12:i:− variants from clinical isolates
| Autor: | Si-Lin Zheng, Bixia Ke, Yu-Wei Jiang, Dong-Mei He, Yang Yu, Jian Sun, Wen-Ying Guo, Xing-Ping Li, Changwen Ke, Ruan-Yang Sun, Ya-Hong Liu, Xiao-Ping Liao, Liang-Xing Fang |
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| Rok vydání: | 2020 |
| Předmět: |
Salmonella typhimurium
0301 basic medicine Microbiology (medical) Serotype China Salmonella Cefotaxime 030106 microbiology Microbial Sensitivity Tests Serogroup medicine.disease_cause 03 medical and health sciences Complete sequence Plasmid medicine Humans Pharmacology (medical) Phylogeny Pharmacology Genetics biology biology.organism_classification Anti-Bacterial Agents 030104 developmental biology Infectious Diseases Salmonella enterica Colistin Mobile genetic elements hormones hormone substitutes and hormone antagonists Plasmids medicine.drug |
| Zdroj: | Journal of Antimicrobial Chemotherapy. 75:1756-1765 |
| ISSN: | 1460-2091 0305-7453 |
| DOI: | 10.1093/jac/dkaa115 |
| Popis: | ObjectivesTo investigate the prevalence and transmission of mcr-3 among Salmonella enterica serotype Typhimurium and 1,4,[5],12:i:−.MethodsA total of 4724 clinical Salmonella isolates were screened for the presence of mcr-3 in China during 2014–19. The clonal relationship of the mcr-3-positive isolates and their plasmid contents and complete sequence were also characterized based on WGS data from the Illumina and MinION platforms.ResultsWe identified 10 mcr-3-positive isolates, and all were MDR, mostly resistant to colistin, cefotaxime, ciprofloxacin, doxycycline and florfenicol. mcr-3 was co-present with blaCTX-M-55-qnrS1 on hybrid ST3-IncC-FII conjugatable plasmids (n = 6) and an ST3-IncC non-conjugatable plasmid (n = 1) and embedded into a pCHL5009T-like IncFII plasmid on the Salmonella chromosome (n = 3). Four distinctive genetic contexts surrounded mcr-3 and all but one were closely related to each other and to the corresponding region of IncFII plasmid pCHL5009T. IS15DI was most likely the vehicle for integration of mcr-3-carrying IncFII plasmids into ST3-IncC plasmids and the chromosome and for shaping the MDR regions. In addition, a phylogenetic tree based on the core genome revealed a unique Salmonella lineage (≤665 SNPs) that contained these 10 mcr-3-positive isolates and another 38 (33 from patients) mcr-3-positive Salmonella from five countries. In particular, most of the 51 mcr-3-positive isolates belonged to ST34 and harboured diverse antibiotic resistance genes (ARGs), including mcr-3-blaCTX-M-55-qnrS1, and possessed similar ARG profiles.ConclusionsOur findings revealed global clonal spread of MDR ST34 Salmonella from clinical isolates co-harbouring mcr-3 with blaCTX-M-55 and qnrS1 and a flexibility of mcr-3 co-transmittance with other ARGs mediated by mobile genetic elements. |
| Databáze: | OpenAIRE |
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