Tick Haller’s Organ, a New Paradigm for Arthropod Olfaction: How Ticks Differ from Insects

Autor: Brooke W. Bissinger, R. Michael Roe, Anirudh Dhammi, Ann L. Carr, Daniel E. Sonenshine, Robert D. Mitchell
Jazyk: angličtina
Rok vydání: 2017
Předmět:
0301 basic medicine
Male
Pathology
Chemoreceptor
Odorant binding
Insect
gustation
lcsh:Chemistry
Haller’s organ
0302 clinical medicine
Ticks
GPCR
lcsh:QH301-705.5
Spectroscopy
Phylogeny
Dermacentor variabilis
media_common
biology
Behavior
Animal
chemoreception
General Medicine
tick
3. Good health
Computer Science Applications
Smell
American dog tick
olfaction
odorant binding proteins
DEET
transcriptome
Taste
Insect Proteins
medicine.medical_specialty
Sensory Receptor Cells
media_common.quotation_subject
Zoology
Olfaction
Tick
Catalysis
Article
Inorganic Chemistry
03 medical and health sciences
medicine
Animals
Amino Acid Sequence
RNA
Messenger
Physical and Theoretical Chemistry
Molecular Biology
Haller's organ
Sequence Analysis
RNA
Organic Chemistry
Animal Structures
biology.organism_classification
030104 developmental biology
lcsh:Biology (General)
lcsh:QD1-999
Arthropod
030217 neurology & neurosurgery
Zdroj: International Journal of Molecular Sciences, Vol 18, Iss 7, p 1563 (2017)
International Journal of Molecular Sciences
International Journal of Molecular Sciences; Volume 18; Issue 7; Pages: 1563
ISSN: 1422-0067
Popis: Ticks are the vector of many human and animal diseases; and host detection is critical to this process. Ticks have a unique sensory structure located exclusively on the 1st pairs of legs; the fore-tarsal Haller’s organ, not found in any other animals, presumed to function like the insect antennae in chemosensation but morphologically very different. The mechanism of tick chemoreception is unknown. Utilizing next-generation sequencing and comparative transcriptomics between the 1st and 4th legs (the latter without the Haller’s organ), we characterized 1st leg specific and putative Haller’s organ specific transcripts from adult American dog ticks, Dermacentor variabilis. The analysis suggested that the Haller’s organ is involved in olfaction, not gustation. No known odorant binding proteins like those found in insects, chemosensory lipocalins or typical insect olfactory mechanisms were identified; with the transcriptomic data only supporting a possible olfactory G-protein coupled receptor (GPCR) signal cascade unique to the Haller’s organ. Each component of the olfactory GPCR signal cascade was identified and characterized. The expression of GPCR, Gαo and β-arrestin transcripts identified exclusively in the 1st leg transcriptome, and putatively Haller’s organ specific, were examined in unfed and blood-fed adult female and male D. variabilis. Blood feeding to repletion in adult females down-regulated the expression of all three chemosensory transcripts in females but not in males; consistent with differences in post-feeding tick behavior between sexes and an expected reduced chemosensory function in females as they leave the host. Data are presented for the first time of the potential hormonal regulation of tick chemosensation; behavioral assays confirmed the role of the Haller’s organ in N,N-diethyl-meta-toluamide (DEET) repellency but showed no role for the Haller’s organ in host attachment. Further research is needed to understand the potential role of the GPCR cascade in olfaction.
Databáze: OpenAIRE
Externí odkaz: