Staphylococcus aureus from 152 cases of bovine, ovine and caprine mastitis investigated by Multiple-locus variable number of tandem repeat analysis (MLVA)

Autor: Michaël Treilles, Christine Pourcel, Stefan Schwarz, Gilles Vergnaud, Andrea T. Feßler, Florence B. Gilbert, Daniel Sobral, Eric Jacquet, Dominique Bergonier, Philippe Bouloc
Přispěvatelé: Interactions hôtes-agents pathogènes [Toulouse] (IHAP), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Institut de génétique et microbiologie [Orsay] (IGM), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), Centre Européen d'Expertise et de Recherche sur les Agents Microbiens (CEERAM), Institute of Farm Animal Genetics (ING), Friedrich-Loeffler-Institut (FLI), Institut de Chimie des Substances Naturelles (ICSN), Centre National de la Recherche Scientifique (CNRS), UR Infectiologie animale et Santé publique (UR IASP), Institut National de la Recherche Agronomique (INRA), Laboratoire Départemental d'Analyses de la Manche (LDA50), École Nationale Supérieure de Techniques Avancées (ENSTA Paris), German Federal Ministry of Education and Research (BMBF) through the German Aerospace Center (DLR), association 'Vaincre La Mucoviscidose', Bergonier, Dominique, Vergnaud, Gilles, Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours, Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT)
Jazyk: angličtina
Rok vydání: 2014
Předmět:
Methicillin-Resistant Staphylococcus aureus
Veterinary medicine
Staphylococcus aureus
bovin
Population
Cattle Diseases
Sheep Diseases
Mastitis
Minisatellite Repeats
Multiple Loci VNTR Analysis
Biology
medicine.disease_cause
Evolution
Molecular
Tandem repeat
ovin
typage de microorganismes
Genotype
medicine
Animals
education
caprin
Alleles
Retrospective Studies
2. Zero hunger
Genetics
bactérie
education.field_of_study
Genetic diversity
Goat Diseases
Sheep
General Veterinary
Research
Goats
Microbiology and Parasitology
Staphylococcal Infections
medicine.disease
bacterial infections and mycoses
veterinary(all)
Microbiologie et Parasitologie
mammite
Variable number tandem repeat
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
Cattle
Female
analyse génétique
Multilocus Sequence Typing
Zdroj: Veterinary Research
Veterinary Research, BioMed Central, 2014, 45 (1), pp.97. ⟨10.1186/s13567-014-0097-4⟩
Veterinary Research 1 (45), 97. (2014)
ISSN: 0928-4249
1297-9716
DOI: 10.1186/s13567-014-0097-4⟩
Popis: Staphylococcus aureus is one of the main etiological agents of mastitis in ruminants. In the present retrospective study, we evaluated the potential interest of a previously described automated multiple loci Variable Number of Tandem Repeats (VNTR) Assay (MLVA) comprising 16 loci as a first line tool to investigate the population structure of S. aureus from mastitis. We determined the genetic diversity of S. aureus strains from cases of clinical and subclinical mastitis in dairy cattle (n = 118, of which 16 were methicillin-resistant), sheep (n = 18) and goats (n = 16). The 152 strains could be subdivided into 115 MLVA genotypes (including 14 genotypes for the ovine strains and 15 genotypes for the caprine strains). This corresponds to a discriminatory index (D) value of 0.9936. Comparison with published MLVA data obtained using the same protocol applied to strains from diverse human and animal origins revealed a low number (8.5%) of human-related MLVA genotypes among the present collection. Eighteen percent of the S. aureus mastitis collection belonged to clonal complexes apparently not associated with other pathological conditions. Some of them displayed a relatively low level of diversity in agreement with a restricted ecological niche. These findings provide arguments suggesting that specific S. aureus lineages particularly adapted to ruminant mammary glands have emerged and that MLVA is a convenient tool to provide a broad overview of the population, owing to the availability via internet of databases compiling published MLVA genotypes. Electronic supplementary material The online version of this article (doi:10.1186/s13567-014-0097-4) contains supplementary material, which is available to authorized users.
Databáze: OpenAIRE
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