LET-99, GOA-1/GPA-16, and GPR-1/2 Are Required for Aster-Positioned Cytokinesis
| Autor: | Carrie R. Cowan, Anthony A. Hyman, Jun Kong, Henrik Bringmann |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Embryo
Nonmammalian Time Factors Aurora B kinase Spindle Apparatus GTP-Binding Protein alpha Subunits Gi-Go Biology Septin General Biochemistry Genetics and Molecular Biology GTP-Binding Proteins Animals Cleavage furrow Caenorhabditis elegans Caenorhabditis elegans Proteins Cytokinesis Agricultural and Biological Sciences(all) Phycoplast Biochemistry Genetics and Molecular Biology(all) Spindle midzone Heterotrimeric GTP-Binding Proteins GTP-Binding Protein alpha Subunits Spindle apparatus Cell biology Midbody RNA Interference CELLBIO Anaphase General Agricultural and Biological Sciences |
| Zdroj: | Current Biology |
| ISSN: | 0960-9822 |
| DOI: | 10.1016/j.cub.2006.11.070 |
| Popis: | SummaryAt anaphase, the mitotic spindle positions the cytokinesis furrow [1]. Two populations of spindle microtubules are implicated in cytokinesis: radial microtubule arrays called asters and bundled nonkinetochore microtubules called the spindle midzone [2–4]. In C. elegans embryos, these two populations of microtubules provide two consecutive signals that position the cytokinesis furrow: The first signal is positioned midway between the microtubule asters; the second signal is positioned over the spindle midzone [5]. Evidence for two cytokinesis signals came from the identification of molecules that block midzone-positioned cytokinesis [5–7]. However, no molecules that are only required for, and thus define, the molecular pathway of aster-positioned cytokinesis have been identified. With RNAi screening, we identify LET-99 [8, 9] and the heterotrimeric G proteins GOA-1/GPA-16 and their regulator GPR-1/2 [10–12] in aster-positioned cytokinesis. By using mechanical spindle displacement, we show that the anaphase spindle positions cortical LET-99, at the site of the presumptive cytokinesis furrow. LET-99 enrichment at the furrow depends on the G proteins. GPR-1 is locally reduced at the site of cytokinesis-furrow formation by LET-99, which prevents accumulation of GPR-1 at this site. We conclude that LET-99 and the G proteins define a molecular pathway required for aster-positioned cytokinesis. |
| Databáze: | OpenAIRE |
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