Optimization of Random-Amplified Polymorphic DNA Producing Amplicons up to 8500 bp and Revealing Intraspecies Polymorphism in Leishmania infantum Isolates
| Autor: | Anastasia Diakou, C.I. Dovas |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Long pcr
Biophysics Polymerase Chain Reaction Biochemistry law.invention chemistry.chemical_compound Species Specificity Polymorphism (computer science) law parasitic diseases Animals Leishmania infantum Molecular Biology Polymerase Polymerase chain reaction DNA Primers Genetics Polymorphism Genetic Base Sequence biology Cell Biology Amplicon biology.organism_classification Molecular biology Random Amplified Polymorphic DNA Technique RAPD chemistry biology.protein DNA |
| Zdroj: | Analytical Biochemistry. 288:195-200 |
| ISSN: | 0003-2697 |
| DOI: | 10.1006/abio.2000.4896 |
| Popis: | In the case of investigation of polymorphism in closely related strains, the highest possible complexity of the patterns obtained by random-amplified polymorphic DNA PCR (RAPD-PCR) is required to assure revealing of limited polymorphism. In the present work, most parameters (reaction components concentration, additives, different polymerases, and thermal profiles) affecting RAPD-PCR were examined, in an effort to increase pattern complexity. A long PCR thermal profile, betaine as cosolvent, and Dynazyme EXT polymerase produced longer amplicons and higher pattern complexity, revealing polymorphism among Leishmania infantum isolates from infected dogs originating from Northern Greece (Macedonia). |
| Databáze: | OpenAIRE |
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