INFLUENCE OF OVARIAN HORMONES ON SPERM 'BREAKAGE' AND DISAPPEARANCE IN SHEEP UTERI
| Autor: | Hawk Hw, Cooper Bs |
|---|---|
| Rok vydání: | 1971 |
| Předmět: |
Male
endocrine system Embryology medicine.medical_specialty media_common.quotation_subject Semen Biology Luteal phase Insemination Andrology Endocrinology Estrus Corpus Luteum Pregnancy Internal medicine medicine Animals Castration Gonadal Steroid Hormones Ligation Ovulation Progesterone reproductive and urinary physiology media_common Estrous cycle Sheep Estradiol urogenital system Ovary Uterus Obstetrics and Gynecology Uterine horns Cell Biology Spermatozoa Sperm Reproductive Medicine Ovariectomized rat Female |
| Zdroj: | Reproduction. 27:349-354 |
| ISSN: | 1741-7899 1470-1626 |
| DOI: | 10.1530/jrf.0.0270349 |
| Popis: | After intrauterine insemination of large animals the endocrine state PR of the female has been shown to influence the fate of the sperm cells. Since factors that influence the fate of sperm cells in the female reproductive tract can affect fertility this study was undertaken to acquire more information concerning hormonal effects on sperm breakage and disappearance in the sheep uterus. Mature parous Rambouillet ewes were used 9 ewes in each of 7 experimental groups. In 45 of the ewes (both ovaries were removed about 2 months before experimental treatment. The other 18 ewes were checked for estrus twice daily by use of vasectomized rams. The ejaculation of seminal fluid into estrous ewes was prevented. Experimental groups were: Group 1 estrous ewes used on the day that heat was detected Group 2 ovariectomized ewes injected with 300 mcg of estradiol-17beta per day for 5 days Group 3 ovariectomized ewes injected with 300 mcg of estradiol-17beta and 5 mg of progesterone per day for 5 days Group 4 ovariectomized ewes injected with 20 mg of progesterone and 5 mcg of estradiol-17beta per day for 5 days and then 20 mg of progesterone and 300 mcg of estradiol-17beta for 1 day followed by 300 mcg of estradil-17beta per day for 20 days Group 5 ovariectomized ewes with no hormonal treatment Group 6 luteal phase ewes used 9 days after estrus and Group 7 ovariectomized ewes injected with 20 mg of progesterone and 5 mcg of estradiol-17beta for 2 days followed by 20 mcg of progesterone per day for 3 days. Semen was deposited in both uterine horms of each ewe after both ends of the horn had been ligated. Less than 2% of the sperm cells used had tailless heads. Ewes were killed 5 hours after insemination. Uterine horns were removed and sperm cells flushed out with saline solution. The numbers of intact tailless and total spermatozoa recovered from the uterus were analyzed statisticaly. From ovariectomized untreated ewes (Group 5) sperm cells deposited in the uterus were recovered intact. Significantly fewer sperm cells were recovered intact from estrous ewes (Group 1) or from any of the ewes treated primarily with estradiol (Groups 2 3 and 4). Greater numbers of intact spermatozoa were recovered from luteal phase or ovariectomized progesterone-treated ewes (Groups 6 and 7) than from any of the other treated groups. Greater numbers of tailless sperm heads were recovered from estrous or ovariectomized estradiol-treated ewes (Groups 1 and 2) than from luteal phase ovariectomized progesterone-treated ewes (Groups 6 and 7). The fewest total sperm were recovered from ovariectomized ewes primarily treated with estriol (Groups 2 3 and 4). The greatest total sperm were recovered from the luteal phase ewes. The disappearance of sperm cells was greatest in ewes treated primarily with estradiol (Groups 2 3 and 4) and least in luteal phase or progesterone-injected ewes (Groups 6 and 7). It is thought that most sperm cells that disappeared from the uterus were phagocytosed. Results indicate that ovarian hormones influenced both the breakage and the disappearance of sperm cells in the sheep uterus. |
| Databáze: | OpenAIRE |
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